UNDER CONSTRUCTION. The links here are being updated to the pages of the new site. Use this part of our website to answer your pombe questions. If you have a question that isn’t answered here, you can try asking pombelist.
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- Make diploids?
- Work with h90 strains?
- Work with non-sporulating (mat2-102) diploids?
- Make crosses?
- Pull tetrads or do random spores?
- Choose markers?
- Do bulk spore germination?
- Do colony PCR?
- Construct disruptions and integrations?
- Find genetic mapping data?
- Make media?
- Counterselect with FOA?
- Use drug selections?
- Just where are all those ade6 mutations, anyway?
- Grow in non-fermentable carbon sources?
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- Do FACS (flow cytometry)?
- Find out why pombe FACS has no 1C peak?
- DAPI stain fixed cells?
- Choose the right plasmid?
- Choose the right promoter?
- Transform pombe with a plasmid?
- Shut off the nmt promoter?
- Titrate expression from the nmt promoter?
- Do cross-complementation with genes from other species?
- Do plasmid shuffle?
- Make a temperature sensitive mutant?
- Use hydroxylamine to mutagenize my plasmid in vitro?
- Find more protocols?
- Find info about GFP fusions?
- Treat my cells with DNA damaging agents?
- Design primers to disrupt or amplify open reading frames?
- Design TAP-tag fusions for protein purifications?
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- Get strains?
- Get plasmids?
- Find references for plasmids and methods?
- Name my gene or contact the nomenclature committee?
- Make drug stocks?
- Perform image analysis / segmentation?
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- Find or analyze sequences?
- Access the pombe genome project?
- Find out about post-genomics? (also see Juerg’s page )
- Find the S. cerevisiae homologue to my gene?
- Find out about genome-wide epigenetic analysis (from Shiv Grewal’s lab)
- Find out genome statistics?
- Find out restriction site usage in the genome?
- Check codon usage?
- Learn about the related yeasts S. japonicus, octosporus, and cryophilus?
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- Find pombe people?
- Find pombe meetings?
- Join mailing lists and newsgroups?
- Find postdoc positions?
- Find the latest community news?