UNDER CONSTRUCTION. The links here are being updated to the pages of the new site. Use this part of our website to answer your pombe questions. If you have a question that isn’t answered here, you can try asking pombelist.

    • What’s pombe?
    • What are some useful numbers and constants about pombe (the almanac)?
    • How evolutionarily distant is pombe from cerevisiae?
    • Can I make beer with pombe?
    • Where can I learn about S. pombe history?
    • Where does wild type strain 972 come from?
    • Do FACS (flow cytometry)?
    • Find out why pombe FACS has no 1C peak?
    • DAPI stain fixed cells?
    • Choose the right plasmid?
    • Choose the right promoter?
    • Transform pombe with a plasmid?
    • Shut off the nmt promoter?
    • Titrate expression from the nmt promoter?
    • Do cross-complementation with genes from other species?
    • Do plasmid shuffle?
    • Make a temperature sensitive mutant?
    • Use hydroxylamine to mutagenize my plasmid in vitro?
    • Find more protocols?
    • Find info about GFP fusions?
    • Treat my cells with DNA damaging agents?
    • Design primers to disrupt or amplify open reading frames?
    • Design TAP-tag fusions for protein purifications?

     

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    • Find or analyze sequences?
    • Access the pombe genome project?
    • Find out about post-genomics? (also see Juerg’s page )
    • Find the S. cerevisiae homologue to my gene?
    • Find out about genome-wide epigenetic analysis (from Shiv Grewal’s lab)
    • Find out genome statistics?
    • Find out restriction site usage in the genome?
    • Check codon usage?
    • Learn about the related yeasts S. japonicus, octosporus, and cryophilus?

     

    • Find pombe people?
    • Find pombe meetings?
    • Join mailing lists and newsgroups?
    • Find postdoc positions?
    • Find the latest community news?