This part of our site includes methods and techniques papers, including those published by our lab (upper section) as well as by others, particularly older papers (lower section).

    • A NAME=”Akman”>Akman, G., and MacNeill, S. MCM-GINS and MCM-MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast. BMC Cell Biology 2009, 10:12
    • Apolinario, E., Nocero, M., Jin, M. and Hoffman, C. S. (1993). Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24:491-495.
    • Bach, M. L. (1987). Cloning and expression of the omp decarboxylase gene ura4 from Schizosaccharomyces pombe. Curr. Genet. 12, 527-534.
    • Bähler J., Wu J.Q., Longtine M.S., Shah N.G., McKenzie A. 3rd, Steever A.B., Wach A., Philippsen P., Pringle J.R. (1998). Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast14: 943-951. You can get the plasmids from Jurg Bähler or from Jianqiu Wu
    • Barbet, N., Muriel, W. J. and Carr, A. M. (1992). Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114:59-66.
    • Basi, G., Schmid, E. and Maundrell, K. (1993). TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 123:131-136.
    • Beach, D., Piper, P. and Nurse, P. (1982). Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation. Mol. Gen. Genet. 187:326-329.
    • Bonfils D, Gmunder H, Lehmann E, Rothlisberger M, Gafner J. (1989) Characterisation of an ARS-plasmid stabilisation element in the fission yeast Schizosaccharomyces pombe. Yeast. 1989 Apr;5 Spec No:S261-6.. The sequence of the fission yeast stb element.
    • Brun, C., Dubey, D. D. and Huberman, J. A. (1995). Pdblet, a Stable Autonomously Replicating Shuttle Vector For Schizosaccharomyces pombe. Gene 164:173-177.
    • Burke, J. D. and Gould, K. L. (1994). Molecular cloning and characterization of the Schizosaccharomyces pombe his3+ gene for use as a selectable marker. Mol Gen Genet 242:169-176.
    • Burland, T. G., Pallotta, D., Tardif, M. C., Lemieux, G. and Dove, W. F. (1991). Fission yeast promoter-probe vectors based on hygromycin resistance. Gene 100 241-245.
    • Chang EC, Barr M, Wang Y, Jung V, Xu HP, Wigler MH (1994). Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Cell 79: 131-141
    • Cottarel G, Beach D, and Deuschle U.(1993) Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23:547-8.
    • Cottarel, G. (1995) The S. cerevisiae HIS3 and LYS2 genes complement that S. pombe his5-303 and lys1-131 mutations, respectively: new selectable markers and new multi-purpose multicopy shuttle vectors, pSP3 and pSP4. Curr. Genet 28: 380-383
    • Craven, R.A., Griffiths, D.J., Sheldrick, K..S, Randall, R.E., Hagan, I.M., Carr, A.M. (1998) Vectors for the expression of tagged proteins in Schizosaccharomyces pombe.Gene 221:59-68
    • Ding DQ, Tomita Y, Yamamoto A, Chikashige Y, Haraguchi T, Hiraoka Y. (2000) Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library. Genes Cells. 5(3):169-190.
    • Ekwall, K and Ruusala, T. (1991) Budding yeast CAN1 gene as a selection marker in fission yeast. Nucl. Acids Res. 19:1150
    • Faryar, K. and Gatz, C. (1992). Construction of a tetracycline-inducible promoter in Schizosaccharomyces pombe. Curr Genet 21:345-349.
    • Forsburg, S. L. (1993). Comparison of different Schizosaccharomyces pombe expression systems. Nucl. Acids Res. 21:2955-2956.
    • Forsburg, S.L. (1994). Codon usage table for Schizosaccharomyces pombe. Yeast 10: 1045-1047
    • Forsburg, S. L. (2001) The art and design of genetic screens: yeast. Nat. Rev. Genet. 2:659-668
    • Forsburg, S.L. (2003). Schizosaccharomyces pombe. In: Current Protocols in Molecular Biology, K. Struhl, section editor. New York: J. Wiley pp. 13.14.1-13.17.7.
    • Forsburg, S.L. and Rhind, N. (2006) Basic methods for fission yeast. Yeast, 23:173-183
    • Forsburg, S.L., and Sherman, D.A. (1997). General purpose tagging vectors for fission yeast. Gene 191: 191-195.
    • Grallert, B., Nurse, P., and Patterson, T. E. (1993). A study of integrative transformation in Schizosaccharomyces pombe. Mol. Gen. Genet. 238, 26-32.
    • Grimm, C., Kohli, J., Murray, J., and Maundrell, K. (1988). Genetic engineering in Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol. Gen. Genet. 215, 81-86.
    • Grimm, C., Bähler, J. and Kohli, J. (1994) M26 recombinational hotspot and physical conversion tract analysis in the ade6 gene of Schizosaccharomyces pombe. Genetics 135 41-51
    • Hindley, J. Phear, G. , Stein, M. and Beach, D. (1987) suc1+ encodes a predicted 13-kd protein that is essential for cell viability and is directly involved in the division cycle of S. pombe. Mol. Cell. Biol. 7:504-511
    • Hodson, J.A., Bailis, J.M, and Forsburg, S. L. (2003). Efficient labeling of fission yeast S. pombe by thymidine and BUdR. Nucl. Acids Res. 31:e134
    • Hoffman, C. S. and Winston, F. (1991). Glucose repression of transcription of the schizosaccharomyces pombe fbp1 gene occurs by a camp signaling pathway. Genes Dev. 5:561-571.
    • Javerzat, J.P., Cranston, G., Allshire, R.C. (1996). Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed. Nucl. Acids Res. 24: 4676-4683
    • Keeney, J. B. and Boeke, J. D. (1994). Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. .Genetics 136:849-856
    • Kiely, J., Haase, S.B., Russell, P., and Leatherwood, J. (2000) Functions of fission yeast orp2+ in DNA replication and checkpointGenetics 154:599-607.
    • Kikuchi, Y., Kitazawa, Y., Shimatake, H., and Yamamoto, M. (1988). The primary structure of the leu1+ gene of Schizosaccharomyces pombe. Curr Genet 14, 375-9.
    • Losson, R., and Lacroute, F. (1983). Plasmids carrying the yeast OMP decarboxy7lase structural and regulatory genes: Transcription regulation in a foreign environment. Cell 32: 371-377.
    • Maundrell, K., Hutchison, A., and Shall, S. (1988). Sequence analysis of ARS elements in fission yeast. EMBO J. 7, 2203-2209.
    • Maundrell, K. (1990). nmt1 of fission yeast: a highly transcribed gene completely repressed by thiamine. J. Biol. Chem. 265:10857-10864.
    • Maundrell, K. (1993). Thiamine-repressible expression vectors prep and prip for fission yeast. Gene 123:127-130.
    • McLeod, M., Stein, M., and Beach, D. (1987) The product of the mei3+ gene expressed under control of the mating type locus, induces meiosis and sporulation in fission yeast. EMBO J. 6:729-736.
    • McNabb, D.S., Pak, S.M., Guarente, L.P. (1997). Cassette for the generation of sequential gene disruptions in the yeast Schizosaccharomyces pombe. Biotechniques 22:1134-1139.
    • Moreno, S., Klar, A., and Nurse, P. (1991). Molecular genetic analysis of the fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823.
    • Moser, M.J., Lee, S.Y., Klevit, R.E., and Davis, T.N. (1995) Ca2+ binding to calmodulin and its role in Schizosaccharomyces pombe as revealed by mutagenesis and NMR spectroscopy. J. Biol. Chem. 270: 20643-52.
    • Nasim, A., Young, P.G., Johnson, B.F (Editors) Molecular Biology of the Fission Yeast (Cell Biology Series) New York: Academic Press (1989)
    • Okazaki, K., Okazaki, N., Kume, K., Jinno, S., Tanaka, K. and Okayama, H. (1990). High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucl. Acids Res. 18:6485-6489.
    • Paluh, J.L., and Clayton, D.A. (1995) Schizosaccharomyces pombe RNase MRP RNA is homologous to metazoan RNase MRP RNAs and may provide clues to interrelationships between RNase MRP and RNase P. Yeast 11:1249-64.
    • Picard, D., Schena, M. and Yamamoto, K. R. (1990). An inducible expression vector for both fission and budding yeast. Gene 86:257-261.
    • Prabhala, G., Rosenberg, G. H., and Kaufer, N. F. (1992). Architectural features of pre-mRNA introns in the fission yeast Schizosaccharomyces pombe. Yeast 8, 171-182.
    • Russell, P. R., and Hall, B. D. (1983). The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe. J. Biol. Chem. 258, 143-149.
    • Russell, P. (1989). Gene cloning and expression in fission yeast, in Molecular Biology of the fission yeast. Nasim, A., Young, P. and Johnson, B. F., eds. Acadmic Press, San Diego. 244-272.
    • Schär, P. and Kohli, J. (1993) Marker effects of G to C transversions on intra- genic recombination and mismatch repair in Schizosaccharomyces pombe. Genetics 133 825-835.
    • Schär, P., Munz, P. and Kohli, J. (1993) Meiotic mismatch repair quantified on the basis of segregation patterns in Schizosaccharomyces pombe. Genetics 133 815-824
    • Siam, R., Dolan, W.P., and Forsburg, S.L.(2004). Choosing and using S pombe plasmids. Methods 33:189-198
    • Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N. In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Methods. 2004 33:213.
    • Snaith HA, Samejima I, Sawin KE. Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast. EMBO J. 2005 Nov 2;24(21):3690-9
    • Szankasi, P., Heyer, W.-D., Schuchert, P., and Kohli, J. (1988). DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe. J. Mol. Biol. 204, 917-925.
    • Tohda, H., Okada, H., Giga-Hama, Y., Okayama, H., and Kumagai, H. (1994) A copy-number controlled expression vector for the fission yeast Schizosaccharomyces pombe. Gene 150:275-280.
    • Waddell, S. and Jenkins, J.R.(1995) arg3+, a new selection marker system for Schizosaccharomyces pombe: application of ura4+ as a removable integration marker. Nucl. Acids Res. 23: 1836-1837.
    • Watt, S, Mata J, López-Maury L, Marguerat S, Burns G, Bähler J. urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. (2008) PLoS ONE. 2008 Jan 16;3(1):e1428.
    • Weilguny, D., Praetorius, M., Carr, A., Egel, R. and Nielsen, O. (1991). New vectors in fission yeast: application for cloning the his2 gene. Gene 99:47-54.
    • Zahn-Zabal M and Kohli J (1996) The distance dependence of the fission yeast ade6-M26 marekr effect in two-factor crosses. Curr Genet 29:530-536
    • M.Q.Zhang and T.G.Marr (1994) Fission yeast gene structure and recognition. Nucl. Acid Res. 22: 1750
    • Zhao, Y., Goriparthi, L., Lieberman, H. B. (1994). A new shuttle vector system for the identification of spontaneous and radiation induced mutations in the fission yeast S. pombe. Mut. Res. 311:111-123.
    • Zhao, Y, Elder RT, Chen M, Cao J (1998). Fission yeast expression vectors adapted for positive identification of gene insertion and green fluorescent protein fusion. BioTechniques 25:438-443