Forsburg Lab Protocols and Recipes

We have written a number of reviews for working in fission yeast:

• Sabatinos, S.A., and Forsburg, S. L. (2010). Molecular Genetics of Schizosaccharomyces pombe Methods Enzymol 470:759-95
• Luche, D.D., and Forsburg, S.L. (2009). Cell cycle synchrony for analysis of S. pombe DNA replication Meth. Mol. Biol 521:437-48.
• Green, M.D., Sabatinos, S.A., and Forsburg, S.L. (2009). Microscopy Techniques to Examine DNA Replication in Fission Yeast Meth. Mol. Biol 521:463-82.
• Sabatinos, S.A., and Forsburg, S.L. (2009). Measuring DNA content by flow cytometry in fission yeast Meth. Mol. Biol 521:449-61.
• Forsburg, S.L. and Rhind, N. (2006) Basic methods for fission yeast. Yeast, 23:173-183
• Siam, R., Dolan, W.P., and Forsburg, S.L. (2004) Choosing and using S. pombe plasmids. Methods 33:189-198.
• Gómez, E.B., and Forsburg, S.L. (2004) Analysis of Schizosaccharomyces pombe cell cycle. Methods Mol. Biol. 241 pp. 93-111
• Forsburg, S. L. 2003. Introduction of DNA into S. pombe cells. Curr Protoc Mol Biol Unit 13 17.
• Forsburg, S. L. 2003. Growth and manipulation of S. pombe. Curr Protoc Mol Biol Unit 13 16.
• Forsburg, S. L. 2003. S. pombe strain maintenance and media. Curr Protoc Mol Biol Unit 13 15.
• Forsburg, S. L. 2003. Overview of Schizosaccharomyces pombe. Curr Protoc Mol Biol Unit 13-14.
• Hodson, J.A., Bailis, J.M. and Forsburg, S.L. (2003) Efficient labeling of fission yeast S. pombe by thymidine and BUdR. Nucl. Acids Res. 31:e134. PMC275491
• Forsburg, S. L. (2001) The art and design of genetic screens: yeast. Nat. Rev. Genet. 2:659-68
• Forsburg, S.L., and Sherman, D.A. (1997). General purpose tagging vectors for fission yeast. Gene 191: 191-195
• Forsburg, S.L. (1994). Codon usage table for Schizosaccharomyces pombe. Yeast 10: 1045-1047
• Forsburg, S.L. (1993). Comparison of different Schizosaccharomyces pombe expression systems. Nucl. Acids Res. 21:2955-2956.PMC309706

Other methods oriented references are collected on the technical references page.