With our proven track records in G protein-coupled receptor and other protein expression and analysis, we offer following services:
-
- Insect cell transfection with bacmid (pFASTbac based vector)
- Small scale expression: 40 ml, 250 ml
- Large scale expression: 1L, 2L, 3L
- Baculovirus generation
Baculovirus expression is done with Sf9 suspension cells. Log phase cells are infected with titered baculovirus. After incubation, protein expression levels are measured with labeled antibody using Guava® easyCyte Flow Cytometers. Through this GPCR specific* assay, we can differentiate expression level on both the cell membrane and inside of cells.
-
- Transfection with purified DNA
- Small scale expression: 30 ml, 200 ml
- Large scale expression: 1L, 2L, 3L
Mammalian expression is done with suspension HEK 293F Suspension Cells, or HEK 293Expi Suspension Cells. Log phase cells are transfected with a CMV based expression plasmid. After incubation, protein expression levels are measured with labeled antibody using Guava® easyCyte Flow Cytometers. Through this GPCR specific *assay, we can differentiate expression level on both the cell membrane and inside of cells.
-
- Weekly and bi-weekly passaging
- Cell banking
- Mycoplasma testing
- Ideal seeding density determination
- Cell stock back ups
- Quality control microscopy and assays to ensure cells are functioning
-
- Cell culture lab set ups
- Cell culture trainings
- Cell culture advisement on projects
- Cell culture protocols
-
(but we can accept any line to bank/maintain, after sterility clearance):
- SF9
- Super SF9
- SF21
- HI-5
- Expi 293F
- HEK-293F
- HEK-293T/17 CR11268
- HEK-293T CRL3216
- HEK-293T CRL1573
- HEK-293S GNTI-
- HEK-293S GNTI- SUPSENSION
- PBC 1.1B4
- PBC 1.2B4
- PBC 1.1E7
- PBC 1.4E7
- PBC HIT-TI5
- Min6
- INS-1E
- NRK
- INS-1E GRINCH (GFP
- IMR-90
- U2OS
- INS-1E 832/13 SERCA2KO (SAKO#8)
- Neuro-2a CCL131
- AtT-20 CCL-89
- AtT-20/D16v-F2 CRL-1795
- INS 832/3 SNAP
Additional Info:
Our services in baculovirus and mammalian expression are comprehensive and can be combined with other services that The Bridge offers, from cloning to expression all the way through to protein purification and quantitation.
Our services are competitive in pricing and our expertise makes for a rapid turnaround time.
All of our data are databased and archived and easily available in excel spreadsheet format.