Development of Digital RT-PCR Methods to Quantify Human-Associated Bacteriophage in Storm Water, Coastal Recreational Waters

Research > Current Projects > Development of Digital RT-­PCR Methods to Quantify Human-­Associated Bacteriophage in Storm Water and Coastal Recreational Waters 
Joshua A. Steele, Microbiologist, Southern California Coastal Water Research Project
Adam C. Martiny, Associate Professor, University of California Irvine, Director UCI OCEANS


Our overall goals are to adapt and further develop a rapid, sensitive water quality monitoring and source-tracking tool that can be used to track the movement of viruses in coastal recreational waters and can be used to track human-associated contamination. Specifically we aim to:
  • Develop a sensitive, robust droplet digital RT-PCR assay to measure and distinguish human-associated and non-human-associated F+RNA coliphage genogroups
  • Apply this assay as microbial source tracking tool in coastal recreational waters and storm waters


Read the summary project proposal

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  • USC Sea Grant
  • 3454 Trousdale Pkwy, CAS 200
  • Los Angeles, CA 90089-0373
  • (213) 740 - 1961