{"id":345,"date":"2023-07-13T12:41:24","date_gmt":"2023-07-13T19:41:24","guid":{"rendered":"https:\/\/live-usc-dornsife.pantheonsite.io\/pombenet\/?page_id=345"},"modified":"2024-08-20T15:38:08","modified_gmt":"2024-08-20T22:38:08","slug":"vector-table","status":"publish","type":"page","link":"https:\/\/dornsife.usc.edu\/pombenet\/vector-table\/","title":{"rendered":"Vector table"},"content":{"rendered":"\n\n \n \n\n\n\n\n\n\n\n
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Search for\u00a0general<\/a>\u00a0purpose,\u00a0expression<\/a>\u00a0vectors,\u00a0tagging\u00a0<\/a>constructs, or\u00a0speciality<\/a>\u00a0plasmids. Send additions!<\/p>\n

General Purpose Fission Yeast Vectors<\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
plasmid name<\/th>\nbase plasmid<\/th>\nyeast markers<\/th>\nyeast origin<\/th>\nother features and comments<\/th>\nrefs\/sources<\/th>\n<\/tr>\n
pAL19<\/td>\npUC<\/td>\nLEU2<\/td>\nars1<\/td>\nblue\/white<\/td>\nT. Carr (at ATCC)<\/td>\n<\/tr>\n
paR3<\/td>\n<\/td>\narg3+<\/td>\nars1<\/td>\n<\/td>\nWaddell<\/td>\n<\/tr>\n
pBG1<\/td>\npUC<\/td>\nhis3+<\/td>\nars1<\/td>\nblue\/white<\/td>\nBurke<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
(sequence)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pDBlet<\/td>\nbluescript<\/td>\nura4+<\/td>\n2x ars3002<\/td>\ndual ars for increased stability<\/td>\nBrun<\/td>\n<\/tr>\n
pDB248X<\/td>\npBR<\/td>\nLEU2<\/td>\n2 micron<\/td>\n<\/td>\nBeach<\/td>\n<\/tr>\n
pEA500<\/td>\npSP2<\/td>\nhis7+<\/td>\nars1<\/td>\n<\/td>\nApolinaro<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFL20<\/td>\npBR322<\/td>\nURA3<\/td>\nars1 stb<\/td>\n<\/td>\nLosson<\/td>\n<\/tr>\n
pIRT2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pIRT2U<\/td>\npUC<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nars1<\/td>\n<\/td>\nHindley<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pIRT2-CAN1<\/td>\npUC<\/td>\nLEU2 CAN1<\/td>\nars1<\/td>\nCAN1 for counter- selection in can1-1 strains<\/td>\nEkwall<\/td>\n<\/tr>\n
pJK148<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pJK210<\/td>\nbluescript<\/td>\nleu1+<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\n—<\/td>\nblue\/white integrating<\/td>\nKeeney<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pON163<\/td>\n<\/td>\nura4+<\/td>\nars1<\/td>\npositive selection for inserts with kanamycin<\/td>\nWeilguny<\/td>\n<\/tr>\n
pNPT\/ADE1-3<\/td>\npART<\/td>\nadh-neoR ade1+<\/td>\nars1<\/td>\ncan be selected for with G418<\/td>\nM. Moser<\/td>\n<\/tr>\n
pSP1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSP2<\/td>\n<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
URA3<\/td>\nars1<\/td>\ncomplements leu1 strains<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
complements ura4 strains<\/td>\nCottarel (a)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSP3<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSP4<\/td>\n<\/td>\nHIS3<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
LYS2<\/td>\nars1<\/td>\ncomplements his5 strains<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
complements lys1 strains<\/td>\nCottarel (b)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pUR18<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pUR19<\/td>\npUC<\/td>\nura4+<\/td>\nars1<\/td>\nblue\/white<\/td>\nBarbet<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pZA57<\/td>\npEA500<\/td>\nhis7+ ade1+<\/td>\nars1<\/td>\npZA58 has ade1+ in opposite orientation<\/td>\nM. Moser<\/td>\n<\/tr>\n
pWH5<\/td>\n<\/td>\nLEU2<\/td>\n2\u00b5<\/td>\nFirst generation vector used in early cloning expts. Now replaced by plasmids such as pAL19, etc<\/td>\nSequence<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Fission Yeast Expression Vectors<\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
name<\/th>\nbase plasmid<\/th>\nyeast markers<\/th>\nyeast origin<\/th>\npromoter<\/th>\nreferences and sources<\/th>\n<\/tr>\n
For more information about promoters, go to the working with pombe page<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pART1<\/td>\npIRT\/pUC<\/td>\nLEU2<\/td>\nars<\/td>\nadh promoter<\/td>\nMcLeod<\/td>\n<\/tr>\n
pCHY21<\/td>\n<\/td>\nURA3<\/td>\nars1<\/td>\nfbp promoter<\/td>\nHoffman<\/td>\n<\/tr>\n
pEVP11<\/td>\n<\/td>\nLEU2<\/td>\n2 \u00b5<\/td>\nadh promoter<\/td>\nRussell<\/td>\n<\/tr>\n
REP1 ,REP3,<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP4<\/td>\npUC<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nars1<\/td>\nnmt1 full strength, Full repression: 15uM thiamine (5ug\/ml). Full induction: no thiamine. Takes about 18 hours. Partial induction: 0.05uM thiamine (0.016ug\/ml). For more information about titrating nmt, and the different levels of expression, visit the working with pombe page.<\/td>\nMaundrell (a), (b)<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Sequences:<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP3<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41,<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42<\/td>\nREP1,2<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nars1<\/td>\nnmt med strength (nmt*)<\/td>\nBasi<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41sequence<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81 ,<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP82<\/td>\nREP1,2<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nars1<\/td>\nnmt low strength (nmt**)<\/td>\nBasi<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81sequence<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
RIP<\/td>\nderived from above: integrating vectors lacking ars1. RIP-s vectors contain sup3-5 on PstI fragment.<\/td>\nMaundrell<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP3X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP4XREP41X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81XREP42X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP82XRIP3X\/s<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
RIP4X\/s<\/td>\nThe REP-X derivatives are derived from the REP\/RIP series above. The original REP\/RIP vectors contain an ATG at the 5′ end of the polylinker; the X-series removes it and adds a XhoI site. Note that you must supply an ATG on your clone if using the X-series.<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Important: the polylinker in the 40-X and 80-X series is not quite the same as the polylinker in the 3X and 4X series. See the map.<\/td>\nForsburg (a)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pYZ1N<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pYZ41N<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pYZ81N<\/td>\nDerived from the REP series, with a lacZ alpha peptide in the polylinker to allow blue\/white selection for inserts.<\/td>\nZhao (b)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF101<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF102<\/td>\npUC<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nars1<\/td>\nCaMV with tet operator, repressed by adh-tet repressor. In the pSLF series, the repressor is provided by integrated pSLF104.<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
In pMLtetON, the repressor is co-expressed on the same plasmid. Use of the analogue anhydrotetracycline at 6uM leads to induction within 3 hrs (max 12hrs). Martin Luetzelberger and Erler et al. (2006; Yeast 23: 813\u2013823)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Martin tells us expression is in the REP41-81 range.<\/td>\nForsburg (a)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF104<\/td>\npUC<\/td>\nsup3-5<\/td>\n—<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pMLtetON<\/td>\n<\/td>\nleu1+; integrate by linearizing with Tth111I or BsiWI<\/td>\nDr Martin Luetzelberger ; sequencehere<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSM1\/2<\/td>\n<\/td>\nLEU2<\/td>\n2 micron<\/td>\nSV40 promoter<\/td>\nRussell<\/td>\n<\/tr>\n
pTLM2\/pAL7 (pAU5)<\/td>\n<\/td>\npSV40-neoR<\/td>\nnone<\/td>\nhCMV promoter; titrating G418 allows increase in copy number<\/td>\nTohda<\/td>\n<\/tr>\n
p2UG<\/td>\n<\/td>\nURA3<\/td>\n2 micron<\/td>\nGRE elements regulated by adh-glucocorticoid receptor (pART)<\/td>\nPicard<\/td>\n<\/tr>\n
pART1\/N795<\/td>\n<\/td>\nLEU2<\/td>\nars1<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSK174<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
and derivatives<\/td>\n<\/td>\nura4+<\/td>\nars1<\/td>\nnmt1-lacO hybrid promoter, co-expresses lacI. Inducible by IPTG in minus thiamine conditions<\/td>\nKjaerulff<\/td>\n<\/tr>\n
There are two commercially available pombe expression systems, from Asahi Glass Co. and Stratagene. This page provides information about them. Obligatory Disclaimer: We are not endorsing any product.<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Tagging \/ Fusion Vectors<\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
plasmid name<\/th>\nbase plasmid<\/th>\nyeast markers<\/th>\npromoter, other features<\/th>\nreferences and source<\/th>\n<\/td>\n<\/tr>\n
Forsburg lab tagging series (compatible polylinkers, modular design)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF172<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF272<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF372<\/td>\nREP4X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP82X<\/td>\nura4+<\/td>\nnmt\/nmt*\/nmt** expression vectors allowing triple HA epitope tag to be fused to C-terminus. Include stop codon, no ATG.<\/td>\nForsburg (b)<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
(at ATCC)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF173<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF273<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF373<\/td>\nREP4X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP82X<\/td>\nura4+<\/td>\nnmt\/nmt*\/nmt** expression vectors allowing triple HA epitope tag to be fused to N-terminus. Contain ATG codon, no stop.<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pDS472<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pDS473<\/td>\nREP4X<\/td>\nura4+<\/td>\nfull strength nmt expression vectors allowing GST tag to be fused to C-terminus (472) or N terminus (473). Compatible with pSLF172\/173 series.<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSGP72<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSGP73<\/td>\nREP3X<\/td>\nLEU2<\/td>\nLEU2 versions of 3xHA tagging vectors pSLF172 and pSLF173 with full strength nmt promoter<\/td>\nS.G. Pasion<\/td>\n<\/td>\n<\/tr>\n
(Forsburg lab)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSGP572<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSGP573<\/td>\nREP4X<\/td>\nura4+<\/td>\nfull strength nmt expression vectors allowing GFP to be fused to C-terminus (572) or N terminus (573). Compatible with pSLF172\/173 series.<\/td>\nPasion<\/td>\n<\/td>\n<\/tr>\n
pSLF972<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pJAH1172<\/td>\nREP4X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP3X<\/td>\nura4+<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
LEU2<\/td>\nfull strength nmt expression vectors allowing 3xV5 tag (from paramyxovirus SV5 p\/k protein) fusion, C terminal only right now. Compatible with pSLF172\/173 series. Antibody to V5 is commercially available from Invitrogen (R960) or Serotec (MCA1360)<\/td>\n(Forsburg lab)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF1072<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pSLF1073<\/td>\nREP4X<\/td>\nura4+<\/td>\nfull strength nmt expression vectors allowing 8XHis HA double tag fusion at C-terminus (1072) or N-terminus (1073). Compatible with pSLF172\/173 series.<\/td>\n(Forsburg lab)<\/td>\n<\/td>\n<\/tr>\n
pNCH1472<\/td>\nREP4X<\/td>\nura4+<\/td>\nfull strength nmt expression vector with 12x myc tag fusion at C-terminus. Compatible with pSLF172\/173 series.<\/td>\n(Forsburg lab)<\/td>\n<\/td>\n<\/tr>\n
Hagan\/Carr tagging series (compatible polylinkers)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41MH-N<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42MH-N<\/td>\nREP41<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nN-terminal tag with 6xHis 2xMyc in mid strength (nmt*) nmt vectors with ura4+ or LEU2 markers<\/td>\nCraven<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41HA-N<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42HA-N<\/td>\nREP41<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nN-terminal tag with 3xHA in mid strength (nmt*) nmt vectors with ura4+ or LEU2 markers<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41Pk-N<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42Pk-N<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81PK-N<\/td>\nREP41<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
LEU2<\/td>\nN-terminal tag with 3xPk tag (from virus SV5 P-protein) in mid strength (nmt*) nmt vectors with ura4+ or LEU2 markers. Antibody to V5 is commercially available from Invitrogen (R960) or Serotec (MCA1360)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41GFP\/EGFP-N<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42GFP\/EGFP-N<\/td>\nREP41<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nN-terminal tag with GFP or enhanced GFP in mid strength (nmt*) nmt vectors with ura4+ or LEU2 markers<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41Pk-C<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42Pk-C<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81Pk-C<\/td>\nREP41<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
LEU2<\/td>\nC-terminal tag with 3xPk tag (from virus SV5 P-protein) in mid strength (nmt*) nmt vectors with ura4+ or LEU2 markers. Antibody to V5 is commercially available from Invitrogen (R960) or Serotec (MCA1360)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41GFP\/EGFP-C<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42GFP\/EGFP-C<\/td>\nREP41<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP42<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nC-terminal tag with GFP or enhanced GFP in mid strength (nmt*) nmt vectors with ura4+ or LEU2 markers<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
B\u00e4hler PCR tagging series<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
KS-ura4<\/td>\nBluescript KS-<\/td>\nura4+<\/td>\nused as a PCR template, followed by transformation for gene deletion<\/td>\nB\u00e4hler et alSequences:<\/td>\n<\/td>\n<\/tr>\n
kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
3HA-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
13Myc-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
GST-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
GFP(S65T)-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
P3nmt1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
P3nmt1-3HA<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
P3nmt1-GFP<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
P3nmt1-GST<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6<\/td>\npFA (Wach et al., 1994, Yeast 10:1793; Wach, 1996, Yeast 12: 259)<\/td>\nkanMX6<\/td>\nused as a PCR template, followed by transformation for gene deletion<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-3HA-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-13Myc-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-GST-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-GFP(S65T)-kanMX6<\/td>\npFA (Wach et al., 1994, Yeast 10:1793; Wach, 1996, Yeast 12: 259)<\/td>\nkanMX6<\/td>\nused as PCR template followed by yeast transformation for C-terminal tagging of proteins by 3HA, 13Myc, GST, or GFP, respectively, at their normal chromosomal locations<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P3nmt1,<\/td>\npFA (Wach et al., 1994, Yeast 10:1793; Wach, 1996, Yeast 12: 259)<\/td>\nkanMX6<\/td>\nused as PCR template followed by yeast transformation for expression of proteins under the nmt1 promoter (3 different strengths) or N-terminal tagging of proteins by 3HA, GST, or GFP, respectively, at their normal chromosomal locations.<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Promoter: nmt1 (full strength: P3nmt1; medium strength: P41nmt1; low strength: P81nmt1)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Others in series: pFA6a-kanMX6-P41nmt1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P81nmt1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P3nmt1-3HA<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P41nmt1-3HA<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P81nmt1-3HA<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P3nmt1-GST<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P41nmt1-GST<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P81nmt1-GST<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P3nmt1-GFP<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P41nmt1-GFP<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-P81nmt1-GFP<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Bahler lab urg1+ promoter-tagging series<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-Purg1<\/td>\npFA (Wach et al., 1994, Yeast 10:1793; Wach, 1996, Yeast 12: 259)<\/td>\nkanMX6<\/td>\nPCR template for expression under urg1+ promoter and\/or N-terminal tagging.<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Others in the series: pFA6a-natMX6-Purg1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-Purg1-3HA<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-Purg1-GST<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX6-Purg1-GFP<\/td>\nWatt et al<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Gould lab TAP-tagging series<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Derived from the B\u00e4hler series. See sequences, maps and links on the Gould lab TAP-tag page<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Also see Juraj Gregan’s Tap Tagging any ORF in pombe page<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Sawin lab red-tagging series<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS390<\/td>\npFA (Wach et al., 1994, Yeast 10:1793; Wach, 1996, Yeast 12: 259)<\/td>\nkanMX6, natMX6<\/td>\nused as PCR template followed by yeast transformation for expression of proteins under the nmt1 promoter (3 different strengths) or N-terminal tagging of proteins at their normal chromosomal locations.<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Promoter: nmt1 (full strength: P3nmt1; medium strength: P41nmt1; low strength: P81nmt1)<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Other plasmids in the series<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS391 pFA6a-mCherry-natMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS392 pFA6a-tdTomato-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS393 pFA6a-tdTomato-natMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS394 pFA6a-kanMX6-P3nmt1-mCherry<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS395 pFA6a-kanMX6-P41nmt1-mCherry<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS396 pFA6a-kanMX6-P81nmt1-mCherry<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS397 pFA6a-kanMX6-P3nmt1-tdTomato<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS398 pFA6a-kanMX6-P41nmt1-tdTomato<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pKS399 pFA6a-kanMX6-P81nmt1-tdTomato<\/td>\nSnaith<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
MacNeill BIFC vectors<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-VN173-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-VN173-natMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-VC155-kanMX6<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-VC155-natMX6<\/td>\npFA (Wach et al., 1994, Yeast 10:1793; Wach, 1996, Yeast 12: 259)<\/td>\nkanMX6, natMX6<\/td>\nused as PCR template followed by yeast transformation<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
These plasmids allow expression of proteins fused to two halves of YFP, allowing for bimolecular fluoresence complementation in vivo. One putative partner needs to be fused to VN173, and one to VC155.<\/td>\nAkman<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Other tagging vectors<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pARTCM<\/td>\npART<\/td>\nLEU2<\/td>\nars1<\/td>\nadh, with N-terminal cMYC tag.<\/td>\nCha
\nng<\/td>\n<\/tr>\n
pALL<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pALU<\/td>\npART<\/td>\nLEU2<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nars1<\/td>\nadh, with N-terminal HA1 tag.<\/td>\nChang<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pYZ3N-GFP<\/td>\nDerived from the REP series, with a lacZ alpha peptide in the polylinker to allow blue\/white selection for inserts and GFP fusion.<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Special Purpose Vectors<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
plasmid name<\/th>\nbase plasmid<\/th>\nyeast markers<\/th>\nyeast origin<\/th>\nother features<\/th>\nreferences and source<\/th>\n<\/tr>\n
pCRR1<\/td>\npBR<\/td>\nLEU2<\/td>\nars1<\/td>\nE. coli markers supF and tetR<\/td>\nZhao<\/td>\n<\/tr>\n
To determine rate of mutagenesis: passage plasmid through pombe and transform into E. coli strain KS40\/pKY241 to quantitate<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pCF83<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pCF85<\/td>\npIRT2U<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pIRT2<\/td>\nura4+<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
LEU2<\/td>\nars1<\/td>\nPromoter reporter construct: S.cerevisaie CYC1 TATA cloned upstream of lacZ<\/td>\nC. Fankhauser<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP3X-lacZ<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41X-lacZ<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81X lacZ<\/td>\nREP3X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP41X<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
REP81X<\/td>\nLEU2<\/td>\nars1<\/td>\nnmt-lacZ reporter fusions<\/td>\nForsburg (a)<\/td>\n<\/td>\n<\/tr>\n
pDM291<\/td>\npSP72<\/td>\nhisG-ura4+-hisG disruption cassette, allowing recovery and re-use of the ura4 marker in disrupted strains<\/td>\nMcNabb<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFS119<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFS118<\/td>\n?<\/td>\nade6+<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
ura4+<\/td>\nars1<\/td>\ncontains adh-thymidine kinase, allowing counterselection with FuDR (see Kiely et al for the method)<\/td>\nSivakumar et al<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pJAH29<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pJAH31<\/td>\npJK148<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pEA2<\/td>\nleu1+<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
his7+<\/td>\nintegration vectors<\/td>\nSystem for labeling cells with BudR. One plasmid expresses adh-tk, and one expresses the hENT nucleoside transporter. These plasmids are also available already integrated into the genome of Forsburg lab strain FY2317<\/td>\nHodson et al<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFS177<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFS181<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFS255<\/td>\npART1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pJK148<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
pFA6a-kanMX<\/td>\nLEU2 ars1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
leu1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
kanR<\/td>\nepisomal adh-hENT1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
integrating adh-hENT1<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
adh1-tk integrating<\/td>\nSystem for labeling cells with BudR. One plasmid expresses adh-tk, and one expresses the hENT nucleoside transporter.<\/td>\nSivakumar et al<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
available fromaddgene<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n<\/div>\n\n\n <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":354,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-345","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nVector table - PombeNet Forsburg Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/dornsife.usc.edu\/pombenet\/vector-table\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Vector table - PombeNet Forsburg Lab\" \/>\n<meta property=\"og:url\" content=\"https:\/\/dornsife.usc.edu\/pombenet\/vector-table\/\" \/>\n<meta property=\"og:site_name\" content=\"PombeNet Forsburg Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2024-08-20T22:38:08+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/dornsife.usc.edu\/pombenet\/vector-table\/\",\"url\":\"https:\/\/dornsife.usc.edu\/pombenet\/vector-table\/\",\"name\":\"Vector table - 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