{"id":264,"date":"2023-07-11T12:59:54","date_gmt":"2023-07-11T19:59:54","guid":{"rendered":"https:\/\/live-usc-dornsife.pantheonsite.io\/pombenet\/?page_id=264"},"modified":"2023-08-03T13:24:36","modified_gmt":"2023-08-03T20:24:36","slug":"protocols","status":"publish","type":"page","link":"https:\/\/dornsife.usc.edu\/pombenet\/protocols\/","title":{"rendered":"Forsburg Lab Protocols and Recipes"},"content":{"rendered":"\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <p>This section of the site has protocols and recipes for working with pombe.<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n\n\n\n                \n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--link-list \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--link-list\"\n    \n      >\n\n    \n      <div class=\"header-container\">\n\n                  \n<div class=\"f--field f--section-title\">\n\n    \n  <h2>\n          Protocols\n      <\/h2>\n\n\n<\/div>\n                <\/div>\n  \n      <div class=\"links-container\">\n\n                        \n        \n          \n            <div class=\"column\">\n                <ul>\n                                                                  <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/transformation\/\n    aria-label=\"Read more about Transformation\"  \n>\n    Transformation \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                                                        <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/dapi-staining\/\n    aria-label=\"Read more about DAPI\/nuclear staining\"  \n>\n    DAPI\/nuclear staining \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                                                        <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/random-spore-analysis\/\n    aria-label=\"Read more about Random Spore Analysis\"  \n>\n    Random Spore Analysis \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                                                        <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/yeast-flow-cytometry\/\n    aria-label=\"Read more about Yeast Flow cytometry\"  \n>\n    Yeast Flow cytometry \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                          <\/ul>\n            <\/div>\n\n          \n          \n        \n          \n            <div class=\"column\">\n                <ul>\n                                                                  <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/telomere-length\/\n    aria-label=\"Read more about Telomere Length\"  \n>\n    Telomere Length \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                                                        <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/hydroxylamine-mutagenesis\/\n    aria-label=\"Read more about Hydroxylamine mutagenesis\"  \n>\n    Hydroxylamine mutagenesis \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                                                        <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/crosses-diploids\/\n    aria-label=\"Read more about Crosses, Diploids and Tetrads\"  \n>\n    Crosses, Diploids and Tetrads \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                                                        <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/disruptions\/\n    aria-label=\"Read more about Disruptions and integrations\"  \n>\n    Disruptions and integrations \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                          <\/ul>\n            <\/div>\n\n          \n          \n        \n\n\n\n    <\/div>\n  \n\n  <\/div><\/div>\n\n\n\n    \n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--link-list \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--link-list\"\n    \n      >\n\n    \n      <div class=\"header-container\">\n\n                  \n<div class=\"f--field f--section-title\">\n\n    \n  <h2>\n          Recipes\n      <\/h2>\n\n\n<\/div>\n                <\/div>\n  \n      <div class=\"links-container\">\n\n                        \n        \n          \n            <div class=\"column\">\n                <ul>\n                                                                  <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/media\/\n    aria-label=\"Read more about Media\"  \n>\n    Media \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                          <\/ul>\n            <\/div>\n\n          \n          \n        \n          \n            <div class=\"column\">\n                <ul>\n                                                                  <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= \/pombenet\/drugs\/\n    aria-label=\"Read more about Drugs\"  \n>\n    Drugs \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                          <\/ul>\n            <\/div>\n\n          \n          \n        \n\n\n\n    <\/div>\n  \n\n  <\/div><\/div>\n\n\n\n\n\n\n  \n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--accordions \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--accordions\"\n    \n      >\n\n    \n  \n      <ul>\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-1-TSAevpG3Bf\" aria-controls=\"section-1-1-TSAevpG3Bf\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">General references<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-1-TSAevpG3Bf\" role=\"region\" aria-labelledby=\"heading-1-1-TSAevpG3Bf\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <div class=\"html-content\">\n<p>We have written a number of reviews for working in fission yeast:<\/p>\n<ul class=\"(null)\">\n<li>Sabatinos, S.A., and\u00a0<strong>Forsburg<\/strong>, S. L. (2010). Molecular Genetics of Schizosaccharomyces pombe\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20946835 | https:\/\/pubmed.ncbi.nlm.nih.gov\/20946835\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20946835\" rel=\"self\">Methods Enzymol 470:759-95<\/a><\/li>\n<li>Luche, D.D., and\u00a0<strong>Forsburg<\/strong>, S.L. (2009). Cell cycle synchrony for analysis of S. pombe DNA replication\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19563121 | https:\/\/pubmed.ncbi.nlm.nih.gov\/19563121\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19563121\" rel=\"self\">Meth. Mol. Biol 521:437-48.<\/a><\/li>\n<li>Green, M.D., Sabatinos, S.A., and\u00a0<strong>Forsburg<\/strong>, S.L. (2009). Microscopy Techniques to Examine DNA Replication in Fission Yeast\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19563123 | https:\/\/pubmed.ncbi.nlm.nih.gov\/19563123\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19563123\" rel=\"self\">Meth. Mol. Biol 521:463-82.<\/a><\/li>\n<li>Sabatinos, S.A., and\u00a0<strong>Forsburg<\/strong>, S.L. (2009). Measuring DNA content by flow cytometry in fission yeast\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19563122 | https:\/\/pubmed.ncbi.nlm.nih.gov\/19563122\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19563122\" rel=\"self\">Meth. Mol. Biol 521:449-61<\/a>.<\/li>\n<li><strong>Forsburg<\/strong>, S.L. and Rhind, N. (2006) Basic methods for fission yeast.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16498704 | https:\/\/pubmed.ncbi.nlm.nih.gov\/16498704\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16498704\" rel=\"self\">Yeast, 23:173-183<\/a><\/li>\n<li>Siam, R., Dolan, W.P., and\u00a0<strong>Forsburg<\/strong>, S.L. (2004) Choosing and using S. pombe plasmids.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/15157885 | https:\/\/pubmed.ncbi.nlm.nih.gov\/15157885\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/15157885\" rel=\"self\">Methods 33:189-198.<\/a><\/li>\n<li>G\u00f3mez, E.B., and\u00a0<strong>Forsburg<\/strong>, S.L. (2004) Analysis of Schizosaccharomyces pombe cell cycle.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/14970648 | https:\/\/pubmed.ncbi.nlm.nih.gov\/14970648\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/14970648\" rel=\"self\">Methods Mol. Biol. 241 pp. 93-111<\/a><\/li>\n<li><strong>Forsburg<\/strong>, S. L. 2003. Introduction of DNA into S. pombe cells.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265321 | https:\/\/pubmed.ncbi.nlm.nih.gov\/18265321\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265321\" rel=\"self\">Curr Protoc Mol Biol Unit 13 17.<\/a><\/li>\n<li><strong>Forsburg<\/strong>, S. L. 2003. Growth and manipulation of S. pombe.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265320 | https:\/\/pubmed.ncbi.nlm.nih.gov\/18265320\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265320\" rel=\"self\">Curr Protoc Mol Biol Unit 13 16.<\/a><\/li>\n<li><strong>Forsburg<\/strong>, S. L. 2003. S. pombe strain maintenance and media.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265319 | https:\/\/pubmed.ncbi.nlm.nih.gov\/18265319\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265319\" rel=\"self\">Curr Protoc Mol Biol Unit 13 15.<\/a><\/li>\n<li><strong>Forsburg<\/strong>, S. L. 2003. Overview of Schizosaccharomyces pombe.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265318 | https:\/\/pubmed.ncbi.nlm.nih.gov\/18265318\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18265318\" rel=\"self\">Curr Protoc Mol Biol Unit 13-14.<\/a><\/li>\n<li>Hodson, J.A., Bailis, J.M. and\u00a0<strong>Forsburg<\/strong>, S.L. (2003) Efficient labeling of fission yeast S. pombe by thymidine and BUdR.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/14576334 | https:\/\/pubmed.ncbi.nlm.nih.gov\/14576334\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/14576334\" rel=\"self\">Nucl. Acids Res. 31:e134.\u00a0<\/a>PMC275491<\/li>\n<li><strong>Forsburg<\/strong>, S. L. (2001) The art and design of genetic screens: yeast.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11533715 | https:\/\/pubmed.ncbi.nlm.nih.gov\/11533715\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11533715\" rel=\"self\">Nat. Rev. Genet. 2:659-68<\/a><\/li>\n<li><strong>Forsburg<\/strong>, S.L., and Sherman, D.A. (1997). General purpose tagging vectors for fission yeast.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/9218719 | https:\/\/pubmed.ncbi.nlm.nih.gov\/9218719\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/9218719\" rel=\"self\">Gene 191: 191-195<\/a><\/li>\n<li><strong>Forsburg<\/strong>, S.L. (1994). Codon usage table for Schizosaccharomyces pombe.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/7992504 | https:\/\/pubmed.ncbi.nlm.nih.gov\/7992504\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/7992504\" rel=\"self\">Yeast 10: 1045-1047<\/a><\/li>\n<li><strong>Forsburg, S.L.<\/strong>\u00a0(1993). Comparison of different Schizosaccharomyces pombe expression systems.\u00a0<a class=\" RLC_Start RLC_Redirect\" title=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/8332516 | https:\/\/pubmed.ncbi.nlm.nih.gov\/8332516\/\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/8332516\" rel=\"self\">Nucl. Acids Res. 21:2955-2956.<\/a>PMC309706<\/li>\n<\/ul>\n<p>Other methods oriented references are collected on the<a class=\" RLC_Start RLC_Ok\" title=\"Technical References\" href=\"\/pombenet\/technical-references\/\" rel=\"self\">\u00a0technical references page.<\/a><\/p>\n<\/div>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n          <\/ul>\n  \n  \n\n  <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":354,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-content-detail.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-264","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Forsburg Lab Protocols and Recipes - PombeNet Forsburg Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/dornsife.usc.edu\/pombenet\/protocols\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Forsburg Lab Protocols and Recipes - 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