{"id":153,"date":"2023-06-28T11:58:38","date_gmt":"2023-06-28T18:58:38","guid":{"rendered":"https:\/\/live-usc-dornsife.pantheonsite.io\/pombenet\/?page_id=153"},"modified":"2023-08-03T13:30:53","modified_gmt":"2023-08-03T20:30:53","slug":"faq","status":"publish","type":"page","link":"https:\/\/dornsife.usc.edu\/pombenet\/faq\/","title":{"rendered":"Pombenet: Frequently Asked Questions"},"content":{"rendered":"\n\n\n\n            \n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--accordions \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--accordions\"\n    \n      >\n\n    \n      <div class=\"header-container\">\n\n      \n                  \n<div class=\"f--field f--description\">\n\n    \n  <p>UNDER CONSTRUCTION. The links here are being updated to the pages of the new site. Use this part of our website to answer your pombe questions. If you have a question that isn&#8217;t answered here, you can try asking pombelist.<\/p>\n\n\n\n<\/div>\n      \n      \n    <\/div>\n  \n      <ul>\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-1-G_ntelWI8F\" aria-controls=\"section-1-1-G_ntelWI8F\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">General<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-1-G_ntelWI8F\" role=\"region\" aria-labelledby=\"heading-1-1-G_ntelWI8F\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <ul>\n<li>What&#8217;s <a href=\"\/pombenet\/about-pombe\/\">pombe<\/a>?<\/li>\n<li>What are some useful numbers and constants about pombe (the <a href=\"\/pombenet\/almanac\/\">almanac<\/a>)?<\/li>\n<li>How evolutionarily <a href=\"\/pombenet\/about-pombe\/\">distant<\/a> is pombe from cerevisiae?<\/li>\n<li>Can I make <a href=\"\/pombenet\/about-pombe\/\">beer<\/a> with pombe?<\/li>\n<li>Where can I learn about S. pombe <a href=\"\/pombenet\/historical-pombe\/\">history<\/a>?<\/li>\n<li>Where does wild type strain <a href=\"\/pombenet\/about-pombe\/\">972<\/a> come from?<\/li>\n<\/ul>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-2-G_ntelWI8F\" aria-controls=\"section-1-2-G_ntelWI8F\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">Genetics: How do I&#8230;.<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-2-G_ntelWI8F\" role=\"region\" aria-labelledby=\"heading-1-2-G_ntelWI8F\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <ul>\n<li>Make <a href=\"\/pombenet\/crosses-diploids\/\">diploids<\/a>?<\/li>\n<li>Work with <a href=\"\/pombenet\/crosses-diploids\/\">h90<\/a> strains?<\/li>\n<li>Work with <a href=\"\/pombenet\/crosses-diploids\/\">non-sporulating<\/a> (mat2-102) diploids?<\/li>\n<li>Make <a href=\"\/pombenet\/crosses-diploids\/\">crosses<\/a>?<\/li>\n<li>Pull <a href=\"\/pombenet\/crosses-diploids\/\">tetrads<\/a> or do random spores?<\/li>\n<li>Choose <a href=\"\/pombenet\/working-with-pombe\/\">markers<\/a>?<\/li>\n<li>Do bulk spore germination?<\/li>\n<li>Do colony PCR?<\/li>\n<li>Construct <a href=\"\/pombenet\/disruptions\/\">disruptions<\/a> and integrations?<\/li>\n<li>Find genetic mapping data?<\/li>\n<li>Make <a href=\"\/pombenet\/media\/\">media<\/a>?<\/li>\n<li>Counterselect with <a href=\"\/pombenet\/drugs\/\">FOA<\/a>?<\/li>\n<li>Use <a href=\"\/pombenet\/drugs\/\">drug selections<\/a>?<\/li>\n<li>Just where are all those <a href=\"\/pombenet\/almanac\/\">ade6<\/a> mutations, anyway?<\/li>\n<li>Grow in non-fermentable <a href=\"\/pombenet\/media\/\">carbon sources<\/a>?<\/li>\n<\/ul>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-3-G_ntelWI8F\" aria-controls=\"section-1-3-G_ntelWI8F\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\"> Cell and Molecular Biology: How Do I&#8230;.<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-3-G_ntelWI8F\" role=\"region\" aria-labelledby=\"heading-1-3-G_ntelWI8F\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <ul>\n<li>Do <a href=\"\/pombenet\/yeast-flow-cytometry\/\">FACS<\/a> (flow cytometry)?<\/li>\n<li>Find out why pombe FACS has no 1C peak?<\/li>\n<li><a href=\"\/pombenet\/dapi-staining\/\">DAPI<\/a> stain fixed cells?<\/li>\n<li>Choose the right <a href=\"\/pombenet\/vectors\/\">plasmid<\/a>?<\/li>\n<li>Choose the right <a href=\"\/pombenet\/working-with-pombe\/\">promoter<\/a>?<\/li>\n<li><a href=\"\/pombenet\/transformation\/\">Transform<\/a> pombe with a plasmid?<\/li>\n<li><a href=\"\/pombenet\/working-with-pombe\/\">Shut off<\/a> the nmt promoter?<\/li>\n<li><a href=\"\/pombenet\/working-with-pombe\/\">Titrate<\/a> expression from the nmt promoter?<\/li>\n<li>Do cross-complementation with genes from other species?<\/li>\n<li>Do <a href=\"\/pombenet\/working-with-pombe\/\">plasmid<\/a> shuffle?<\/li>\n<li>Make a temperature sensitive mutant?<\/li>\n<li>Use hydroxylamine to mutagenize my plasmid in vitro?<\/li>\n<li>Find more protocols?<\/li>\n<li>Find info about GFP fusions?<\/li>\n<li>Treat my cells with <a href=\"\/pombenet\/drugs\/\">DNA damaging agents<\/a>?<\/li>\n<li>Design primers to disrupt or amplify open reading frames?<\/li>\n<li>Design TAP-tag fusions for protein purifications?<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-4-G_ntelWI8F\" aria-controls=\"section-1-4-G_ntelWI8F\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">Reagents, Methods, and References: How Do I&#8230;.<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-4-G_ntelWI8F\" role=\"region\" aria-labelledby=\"heading-1-4-G_ntelWI8F\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <ul>\n<li>Get strains?<\/li>\n<li>Get plasmids?<\/li>\n<li>Find references for <a href=\"\/pombenet\/vector-table\/\">plasmids<\/a> and <a href=\"\/pombenet\/technical-references\/\">methods<\/a>?<\/li>\n<li><a href=\"\/pombenet\/working-with-pombe\/\">Name my gene<\/a> or contact the nomenclature committee?<\/li>\n<li>Make <a href=\"\/pombenet\/drugs\/\">drug stocks<\/a>?<\/li>\n<li>Perform image analysis \/ <a href=\"\/pombenet\/pombex\/\">segmentation<\/a>?<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-5-G_ntelWI8F\" aria-controls=\"section-1-5-G_ntelWI8F\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">Genomics: How Do I&#8230;.<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-5-G_ntelWI8F\" role=\"region\" aria-labelledby=\"heading-1-5-G_ntelWI8F\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <ul>\n<li>Find or analyze sequences?<\/li>\n<li>Access the pombe genome project?<\/li>\n<li>Find out about post-genomics? (also see Juerg&#8217;s page )<\/li>\n<li>Find the S. cerevisiae homologue to my gene?<\/li>\n<li>Find out about genome-wide epigenetic analysis (from Shiv Grewal&#8217;s lab)<\/li>\n<li>Find out genome <a href=\"\/pombenet\/almanac\/\">statistics<\/a>?<\/li>\n<li>Find out <a href=\"\/pombenet\/almanac\/\">restriction site usage<\/a> in the genome?<\/li>\n<li>Check codon <a href=\"\/pombenet\/almanac\/\">usage<\/a>?<\/li>\n<li>Learn about the <a href=\"https:\/\/www.broadinstitute.org\/scientific-community\/science\/projects\/fungal-genome-initiative\/schizosaccharomyces-genomes-project\">related yeasts<\/a> S. japonicus, octosporus, and cryophilus?<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-6-G_ntelWI8F\" aria-controls=\"section-1-6-G_ntelWI8F\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\"> Community: How Do I&#8230;.<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-6-G_ntelWI8F\" role=\"region\" aria-labelledby=\"heading-1-6-G_ntelWI8F\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <ul>\n<li>Find pombe people?<\/li>\n<li>Find pombe meetings?<\/li>\n<li>Join mailing lists and newsgroups?<\/li>\n<li>Find postdoc positions?<\/li>\n<li>Find the latest community news?<\/li>\n<\/ul>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n          <\/ul>\n  \n  \n\n  <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":354,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-153","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Pombenet: Frequently Asked Questions - PombeNet Forsburg Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/dornsife.usc.edu\/pombenet\/faq\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Pombenet: Frequently Asked Questions - PombeNet Forsburg Lab\" \/>\n<meta property=\"og:url\" content=\"https:\/\/dornsife.usc.edu\/pombenet\/faq\/\" \/>\n<meta property=\"og:site_name\" content=\"PombeNet Forsburg Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2023-08-03T20:30:53+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/dornsife.usc.edu\/pombenet\/faq\/\",\"url\":\"https:\/\/dornsife.usc.edu\/pombenet\/faq\/\",\"name\":\"Pombenet: Frequently Asked Questions - PombeNet Forsburg Lab\",\"isPartOf\":{\"@id\":\"https:\/\/dornsife.usc.edu\/pombenet\/#website\"},\"datePublished\":\"2023-06-28T18:58:38+00:00\",\"dateModified\":\"2023-08-03T20:30:53+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/dornsife.usc.edu\/pombenet\/faq\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/dornsife.usc.edu\/pombenet\/faq\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/dornsife.usc.edu\/pombenet\/faq\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/dornsife.usc.edu\/pombenet\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Pombenet: Frequently Asked Questions\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/dornsife.usc.edu\/pombenet\/#website\",\"url\":\"https:\/\/dornsife.usc.edu\/pombenet\/\",\"name\":\"PombeNet Forsburg Lab\",\"description\":\"USC Dornsife PombeNet Forsburg Lab\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/dornsife.usc.edu\/pombenet\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Pombenet: Frequently Asked Questions - PombeNet Forsburg Lab","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/dornsife.usc.edu\/pombenet\/faq\/","og_locale":"en_US","og_type":"article","og_title":"Pombenet: Frequently Asked Questions - PombeNet Forsburg Lab","og_url":"https:\/\/dornsife.usc.edu\/pombenet\/faq\/","og_site_name":"PombeNet Forsburg Lab","article_modified_time":"2023-08-03T20:30:53+00:00","twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/dornsife.usc.edu\/pombenet\/faq\/","url":"https:\/\/dornsife.usc.edu\/pombenet\/faq\/","name":"Pombenet: Frequently Asked Questions - PombeNet Forsburg Lab","isPartOf":{"@id":"https:\/\/dornsife.usc.edu\/pombenet\/#website"},"datePublished":"2023-06-28T18:58:38+00:00","dateModified":"2023-08-03T20:30:53+00:00","breadcrumb":{"@id":"https:\/\/dornsife.usc.edu\/pombenet\/faq\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/dornsife.usc.edu\/pombenet\/faq\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/dornsife.usc.edu\/pombenet\/faq\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/dornsife.usc.edu\/pombenet\/"},{"@type":"ListItem","position":2,"name":"Pombenet: Frequently Asked Questions"}]},{"@type":"WebSite","@id":"https:\/\/dornsife.usc.edu\/pombenet\/#website","url":"https:\/\/dornsife.usc.edu\/pombenet\/","name":"PombeNet Forsburg Lab","description":"USC Dornsife PombeNet Forsburg Lab","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/dornsife.usc.edu\/pombenet\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/pages\/153","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/users\/354"}],"replies":[{"embeddable":true,"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/comments?post=153"}],"version-history":[{"count":9,"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/pages\/153\/revisions"}],"predecessor-version":[{"id":766,"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/pages\/153\/revisions\/766"}],"wp:attachment":[{"href":"https:\/\/dornsife.usc.edu\/pombenet\/wp-json\/wp\/v2\/media?parent=153"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}