{"id":300,"date":"2023-12-01T14:18:01","date_gmt":"2023-12-01T22:18:01","guid":{"rendered":"https:\/\/live-usc-dornsife.pantheonsite.io\/nanobiophysicscore\/?page_id=300"},"modified":"2023-12-07T10:34:11","modified_gmt":"2023-12-07T18:34:11","slug":"microplate-reader","status":"publish","type":"page","link":"https:\/\/dornsife.usc.edu\/nanobiophysicscore\/microplate-reader\/","title":{"rendered":"Synergy H4 Hybrid Multi-Mode Microplate Reader"},"content":{"rendered":"\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <p><strong><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-303\" src=\"\/nanobiophysicscore\/wp-content\/uploads\/sites\/269\/2023\/12\/Synergy_H4_Microplate_Reader-300x291.jpg\" alt=\"Microplate Reader\" width=\"300\" height=\"291\" srcset=\"https:\/\/dornsife.usc.edu\/nanobiophysicscore\/wp-content\/uploads\/sites\/269\/2023\/12\/Synergy_H4_Microplate_Reader-300x291.jpg 300w, https:\/\/dornsife.usc.edu\/nanobiophysicscore\/wp-content\/uploads\/sites\/269\/2023\/12\/Synergy_H4_Microplate_Reader.jpg 396w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>All You Need in One Compact Instrument<\/strong><\/p>\n<p>BioTek Synergy H4 covers the broadest range of applications. Equipped with two main optical systems (monochromater-based and filter-based) and 6 major detection modes (UV-Visible absorbance, top\/bottom fluorescence, luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), and Alpha) it can run virtually any microplate-based assay from basic research assays to high throughput drug discovery assays.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Hybrid Technology: Sensibility and Flexibility<\/strong><\/p>\n<p>Hybrid Technology BioTek&#8217;s patented design that combines two different optical systems in one compact instrument. This design makes Synergy H4 the ideal instrument for research and drug discovery applications when having to choose between flexibility and performance is not an option.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Alpha Detection Mode<\/strong><\/p>\n<p>Alpha-based assays (AlphaScreen, AlphaLISA, SureFire) are growing in popularity because of the workflow simplicity (homogeneous, no-wash assays), and the level of sensitivity they provide for cellular assays. With its unique optical system, Synergy H4 is the optimal reader to provide high-performance detection for Alpha-based assays.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Detection modes:<\/strong><\/p>\n<ul>\n<li>Fluorescence Intensity<\/li>\n<li>Fluorescence Polarization (FP)<\/li>\n<li>Time-Resolved Fluorescence TRF)<\/li>\n<li>Luminescence<\/li>\n<li>UV-Visible Absorbance<\/li>\n<li>AlphaScreen\/ AlphaLISA<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Typical applications:<\/strong><\/p>\n<ul>\n<li>Nucleic acid quantification<\/li>\n<li>Protein quantification<\/li>\n<li>Enzyme kinetics<\/li>\n<li>Biomarker quantification<\/li>\n<li>ELISAs<\/li>\n<li>Genetics analysis<\/li>\n<li>Drug discovery<\/li>\n<li>Cell proliferation<\/li>\n<li>Cytotoxicity<\/li>\n<li>Drug absorption and metabolism<\/li>\n<li>Biologics drug discovery and development<\/li>\n<li>Food safety<\/li>\n<li>Biofuels research<\/li>\n<li>Environmental monitoring<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Specifications:<\/strong><\/p>\n<p><strong>Absorbance<\/strong><\/p>\n<ul>\n<li>Wavelength selection Monochromator<\/li>\n<li>Wavelength range 230 \u0336 999 nm, 1 nm increment<\/li>\n<li>Bandpass 2 nm (230 \u0336 285 nm), 4 nm (&gt;285 nm)<\/li>\n<li>Dynamic range 0 \u0336 4.0 OD<\/li>\n<li>Resolution 0.0001 OD<\/li>\n<li>Path length correction Yes<\/li>\n<li>Reading speed<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 96 well plate: 11 seconds<\/p>\n<p>o\u00a0\u00a0 384 well plate: 22 seconds<\/p>\n<p><strong>Fluorescence Intensity<\/strong><\/p>\n<ul>\n<li>Sensitivity Monochromators:<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Fluorescein 2 pM typical (0.2 fmol\/well 384-well plate) \u2013 Top<\/p>\n<p>o\u00a0\u00a0 Fluorescein 2.5 pM typical (0.25 fmol\/well 384-well plate) \u2013 Bottom<\/p>\n<ul>\n<li>Sensitivity Filters\/mirrors:<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Fluorescein 1 pM typical (0.1 fmol\/well 384-well plate)<\/p>\n<ul>\n<li>Light sources<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Tungsten Halogen lamp<\/p>\n<p>o\u00a0\u00a0 High energy Xenon flash lamp<\/p>\n<ul>\n<li>Wavelength selection<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Double grating monochromators and,<\/p>\n<p>o\u00a0\u00a0 Deep blocking bandpass filters\/dichroic mirrors<\/p>\n<ul>\n<li>Wavelength range<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Monochromators: 250 \u0336 900 nm<\/p>\n<p>o\u00a0\u00a0 Filters: 200 \u0336 700 nm (900 nm option)<\/p>\n<ul>\n<li>Bandpass<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Monochromators: variable 9, 13.5, 17, 20 nm<\/p>\n<p>o\u00a0\u00a0 Filters: filter-dependent, from 5 nm to &gt;100 nm<\/p>\n<ul>\n<li>Detection system<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Red shifted PMT for monochromator-based system<\/p>\n<p>o\u00a0\u00a0 Low Noise PMT for filter-based system<\/p>\n<ul>\n<li>Dynamic range<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 Monochromators: 5 decades<\/p>\n<p>o\u00a0\u00a0 Filters\/mirrors: &gt;6 decades<\/p>\n<ul>\n<li>Reading speed<\/li>\n<\/ul>\n<p>o\u00a0\u00a0 96 well plate: 11 seconds<\/p>\n<p>o\u00a0\u00a0 384 well plate: 22 seconds<\/p>\n<p>o\u00a0\u00a0 1536 well plate: 43 seconds<\/p>\n<p>Luminescence<\/p>\n<ul>\n<li>Sensitivity 10 amol ATP typical (flash)<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Fluorescence Polarization<\/strong><\/p>\n<ul>\n<li>Light source Tungsten Halogen<\/li>\n<li>Sensitivity 3 mP at 1 nM fluorescein typical<\/li>\n<li>Wavelength selection Deep blocking bandpass filters\/dichroic mirrors<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Time-Resolved Fluorescence<\/strong><\/p>\n<ul>\n<li>Light source High energy Xenon flash<\/li>\n<li>Sensitivity Europium 60 fM typical with filters (6 amol\/well in 384-well plate)<\/li>\n<\/ul>\n<p>Manual and presentations are available.\u00a0 <a href=\"\/nanobiophysicscore\/wp-content\/uploads\/sites\/269\/2023\/12\/SynergyH4__Demo_Presentation.ppt\" target=\"_blank\" rel=\"noopener\">SynergyH4__Demo<\/a>, <a href=\"\/nanobiophysicscore\/wp-content\/uploads\/sites\/269\/2023\/12\/Synergy_H4_Applications.pptx\" target=\"_blank\" rel=\"noopener\">Synergy H4 Applications<\/a> and <a href=\"\/nanobiophysicscore\/wp-content\/uploads\/sites\/269\/2023\/12\/Gen_5_v2.0_RevC.pdf\" target=\"_blank\" rel=\"noopener\">Gen5\u2122 Getting Started Guide<\/a>.<\/p>\n<p><strong>For more information go to <\/strong><a href=\"http:\/\/www.biotek.com\/\" target=\"_blank\" rel=\"noopener\">www.biotek.com<\/a><\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":11,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-300","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - 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