{"id":156,"date":"2023-10-19T10:20:30","date_gmt":"2023-10-19T17:20:30","guid":{"rendered":"https:\/\/live-usc-dornsife.pantheonsite.io\/manahan-lab\/?page_id=156"},"modified":"2023-11-13T09:34:46","modified_gmt":"2023-11-13T17:34:46","slug":"laboratory-based-research-activities","status":"publish","type":"page","link":"https:\/\/dornsife.usc.edu\/manahan-lab\/home\/laboratory-based-research-activities\/","title":{"rendered":"Laboratory-based Research Activities"},"content":{"rendered":"\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-204 alignright\" src=\"\/manahan-lab\/wp-content\/uploads\/sites\/277\/2023\/10\/AHF-1.jpg\" alt=\"Photo of building on USC campus\" width=\"240\" height=\"180\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-203 alignleft\" src=\"\/manahan-lab\/wp-content\/uploads\/sites\/277\/2023\/10\/LAB-2.jpg\" alt=\"Lab workers in white coats working in the lab\" width=\"240\" height=\"180\" \/> Our primary laboratory is located on USC&#8217;s University Park Campus.\u00a0 We work in the Allan Hancock Foundation Building of USC&#8217;s <a href=\"https:\/\/dornsife.usc.edu\/bisc\/\" target=\"_blank\" rel=\"noopener\">Department of Biological Sciences<\/a>, where the laboratories of the faculty in the Marine and Environmental Biology research group are located.<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n\n\n\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <h2>Examples of laboratory results in physiology, biochemistry, molecular biology:<\/h2>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-233 alignleft\" src=\"\/manahan-lab\/wp-content\/uploads\/sites\/277\/2023\/10\/RESPIR-300x188.jpg\" alt=\"A line graph measuring respiration. The line goes up.\" width=\"300\" height=\"188\" srcset=\"https:\/\/dornsife.usc.edu\/manahan-lab\/wp-content\/uploads\/sites\/277\/2023\/10\/RESPIR-300x188.jpg 300w, https:\/\/dornsife.usc.edu\/manahan-lab\/wp-content\/uploads\/sites\/277\/2023\/10\/RESPIR.jpg 371w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><strong>Physiology<\/strong><\/h3>\n<p>Respiration rate at 15 deg. C during development of the purpse sea urchin,\u00a0<em>Strongylocentrotus purpuratus<\/em>, from the embryonic to the pluteus larval stage.<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n\n\n\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <h3 style=\"padding-left: 40px; text-align: left;\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-236 alignleft\" src=\"\/manahan-lab\/wp-content\/uploads\/sites\/277\/2023\/10\/2D-GEL.gif\" alt=\"Scatter plot of second dimension separation\" width=\"240\" height=\"215\" \/><strong>Biochemistry<\/strong><\/h3>\n<p style=\"text-align: left;\">Newly-synthesized proteins (35S-Met\/Cys labeled) in larvae of the oyster,\u00a0<em>Crassostrea gigas,<\/em>\u00a0separated by two-dimensional gel electrophoresis.<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n\n\n\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <h3><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-237 alignleft\" src=\"\/manahan-lab\/wp-content\/uploads\/sites\/277\/2023\/10\/TRANS-2-300x231.gif\" alt=\"Diagram  showing the deduced amino acid sequence from a cloned cDNA. Proposed structure of the transporter molecule has 12 membrane-spanning domains.\" width=\"300\" height=\"231\" \/><strong>Molecular Biology<\/strong><\/h3>\n<p>Amino acid transporter in 4-day-old embryos of the temperate sea urchin\u00a0<em>Strongylocentrotus purpuratus<\/em>. Diagram below shows the deduced amino acid sequence from a cloned cDNA. Proposed structure of the transporter molecule has 12 membrane-spanning domains.<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n\n\n  \n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--contact-details \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--contact-details\"\n    \n      >\n\n    \n  <div class=\"inner-wrapper\">\n\n          <div class=\"header-container\">\n\n                      \n<div class=\"f--field f--section-title\">\n\n    \n  <h2>\n          Contact\n      <\/h2>\n\n\n<\/div>\n        \n                      \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/dornsife.usc.edu\/profile\/donal-manahan\/\n  target=\"_blank\"  aria-label=\"Read more about Faculty Profile\"  \n>\n    Faculty Profile \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n        \n      <\/div>\n    \n    \n      <div class=\"contacts-container\">\n\n        \n                      <div class=\"contact-card\">\n\n                              <h3>Professor Donal T. Manahan<\/h3>\n              \n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>Department of Biological Sciences<br \/>\nUSC Dornsife College of Letters, Arts and Sciences<br \/>\nUniversity of Southern California<br \/>\nLos Angeles, CA 90089-0371<\/p>\n<p>&nbsp;<\/p>\n\n\n\n<\/div>\n              \n                              <div class=\"f--phone\">\n                  <a href=\"tel:(213) 740 - 5973\">(213) 740 &#8211; 5973<\/a>\n                <\/div>\n              \n                              <div class=\"f--email\">\n                  <a href=\"mailto:manahan@usc.edu\">manahan@usc.edu<\/a>\n                <\/div>\n              \n            <\/div>\n                    \n      <\/div>\n\n        \n     \n    \n  <\/div>\n\n\n  <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":17,"featured_media":0,"parent":4,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-156","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - 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