Tag Sequencing Protocol with Mock Community Description, from 2016. NOTE:THE COMBINATORIAL BARCODING AND INDEXING STRATEGY HERE IS SUITABLE FOR MISEQ AND HISEQ2500, BUT DUE TO HIGHER INCIDENCE OF INDEX HOPPING WITH NOVASEQ AND OTHER NEW PLATFORMS WITH PATTERNED FLOWCELLS, IT IS HIGHLY RECOMMENDED TO CHANGE TO UNIQUE DUAL INDEXING (UDI). This allows informatic removal of spurious forward-reverse combinations, resembling cross-contamination between samples (up to a few percent of sequences in our experience). We recommend replacing the forward and reverse barcodes/indices with unique 10 nt index combinations.
Filtering for cell collection
Bacterial heterotrophic production
Molecular fingerprinting (ARISA)
Pulse field gel electrophoresis
Rohan Sachdeva; Cheryl Chow
Tutorial: Cytoscape-- Visualization of Microbial Networks
Cheryl Chow/ Jacob Cram