{"id":443,"date":"2023-10-11T16:34:23","date_gmt":"2023-10-11T23:34:23","guid":{"rendered":"https:\/\/dornsife.usc.edu\/fuhrmanlab\/?page_id=443"},"modified":"2024-07-29T11:42:39","modified_gmt":"2024-07-29T18:42:39","slug":"research","status":"publish","type":"page","link":"https:\/\/dornsife.usc.edu\/fuhrmanlab\/research\/","title":{"rendered":"Research"},"content":{"rendered":"\n\n\n  \n              \n    \n    \n              \n    \n    \n        \n    \n    \n  \n    \n    \n  \n    \n    \n  \n    \n    \n  \n    \n  \n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--interactive-content-pane \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--interactive-content-pane\"\n    \n      >\n\n    \n<div class=\"inner-wrapper\">\n  <div class=\"left\">\n              \n<div class=\"f--field f--section-title\">\n\n    \n  <h2>\n          Ongoing Projects\n      <\/h2>\n\n\n<\/div>\n      \n    <div class=\"pagination-wrapper\">\n      <ul class=\"interactive-content-pane-pagination\"><\/ul>\n    <\/div>\n  <\/div>\n\n  <div class=\"right\">\n          <div class=\"swiper\">\n        <div class=\"swiper-wrapper\">\n                      <div class=\"swiper-slide\">\n              <h3 class=\"pane-title\">\n                San Pedro Ocean Time Series and Microbial Observatory\n              <\/h3>\n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>The San Pedro Ocean Time-series (SPOT) is an ongoing monthly sampling of oceanographic data from the San Pedro Channel, about 20 km offshore and roughly midway between Los Angeles and Santa Catalina Island, supported by the Wrigley Institute and NSF. The Fuhrman lab has been studying the ecological patterns of the microbial community abundance, activity, and composition at SPOT since 2000. The site depth is 900 m, and for our work, samples are routinely collected from 5 m, the chlorophyll maximum depth (typically 15-45 m), 150 m, 500 m, and 890 m. The location has strong influence from the open ocean, but seasonally is influenced by upwelling elsewhere in the region. Samples at 500m and deeper are low in oxygen content, and very low near the sea floor. Productivity and heterotrophic activity peak in spring, and in late summer and fall the microbial community in the euphotic zone strongly resembles the adjacent oligotrophic open ocean.\u00a0 The top two depths show a remarkably steady <em>long-term average<\/em> prokaryotic and viral community composition, with superimposed strong repeating seasonality. The phytoplankton community has more variability than the prokaryotes, but is also moderately predictable and seasonal. The heterotrophic protists are the least predictable.<\/p>\n\n\n\n<\/div>\n                          \n  \n                              <ul>\n                                            <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/dornsife.usc.edu\/spot\n  target=\"_blank\"  aria-label=\"Read more about Wrigley SPOT program\"  \n>\n    Wrigley SPOT program \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#FC0\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n                        <\/li>\n                                            <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/dornsife.usc.edu\/fuhrmanlab\/research\/usc-microbial-observatory\/\n  target=\"_blank\"  aria-label=\"Read more about Fuhrman lab SPOT Microbial Observatory\"  \n>\n    Fuhrman lab SPOT Microbial Observatory \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#FC0\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n                        <\/li>\n                                    <\/ul>\n                          <\/div>\n                      <div class=\"swiper-slide\">\n              <h3 class=\"pane-title\">\n                Global Marine Biogeographies through CBIOMES\n              <\/h3>\n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>The Simons Collaboration on Computational Biogeochemical Modeling of Marine Ecosystems (CBIOMES) seeks to develop and apply quantitative models of the structure and function of marine microbial communities at seasonal and basin scales. Our CBIOMES project focus is on microbial growth, interactions and biogeographies from \u2018omics data. We collaborate strongly with other CBIOMES investigators, most often on the biogeographic distributions of phytoplankton and other microorganisms in the global ocean. Our most significant contribution is our assessment of the distributions of archaea, bacteria, and eukaryotes on large ocean transects by means of universal 3-domain rRNA sequence analysis.<\/p>\n\n\n\n<\/div>\n                          \n  \n                              <ul>\n                                            <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/cbiomes.org\/\n  target=\"_blank\"  aria-label=\"Read more about CBIOMES\"  \n>\n    CBIOMES \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#FC0\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n                        <\/li>\n                                            <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/dornsife.usc.edu\/fuhrmanlab\/research\/cbiomes\/\n  target=\"_blank\"  aria-label=\"Read more about Fuhrman lab CBIOMES work\"  \n>\n    Fuhrman lab CBIOMES work \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#FC0\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n                        <\/li>\n                                    <\/ul>\n                          <\/div>\n                      <div class=\"swiper-slide\">\n              <h3 class=\"pane-title\">\n                515\/916 Universal Primer Validation\n              <\/h3>\n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>Our 515-926 primer pair not only amplifies the ssu rRNA (16S or 18S) of the vast majority of known organisms in all three domains, but testing with our prokaryotic mock communities shows that the results are remarkably quantitative.<\/p>\n<p>For a set of recommendations on how to use the Fuhrman Lab\u2019s mock communities and PCR primers , please see our <a href=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/methods\/\" target=\"_blank\" rel=\"noopener\">Methods<\/a> or selected publications: <a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.13023\/abstract\">Parada et al. 2015<\/a>, <a href=\"http:\/\/www.nature.com\/articles\/nmicrobiol20165\">Needham and Fuhrman 2016<\/a>, <a title=\"Yeh et al 2018\" href=\"https:\/\/doi.org\/10.1128\/mSystems.00023-18\">Walters et al. 2016, Yeh et al 2018.<\/a><\/p>\n\n\n\n<\/div>\n                          \n  \n                              <ul>\n                                            <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/dornsife.usc.edu\/fuhrmanlab\/methods\/\n    aria-label=\"Read more about link to primers protocol\"  \n>\n    link to primers protocol \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#FC0\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n                        <\/li>\n                                    <\/ul>\n                          <\/div>\n                      <div class=\"swiper-slide\">\n              <h3 class=\"pane-title\">\n                Virus\/Host Community Dynamics\n              <\/h3>\n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>Bacterial and archaeal populations are shaped by resource availability as well as viral lysis and protist grazing. Bottom-up and top-down controls are ultimately equally important. We use dilution experiments, time-series data, network analysis, and statistical tools to simultaneously assess top-down and bottom-up regulation on community-wide abundance and diversity.<\/p>\n<p>Selected publications: <a href=\"https:\/\/doi.org\/10.3390\/v15020581\">Dart, et al. 2023<\/a>, <a href=\"https:\/\/www.nature.com\/articles\/s41564-019-0628-x\">Ignacio-Espinoza, et al. 2019<\/a>, <a href=\"https:\/\/ami-journals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.14687\">Ahlgren, et al. 2019<\/a>, <a title=\"Sieradzki et al 2019\" href=\"https:\/\/doi.org\/10.1038\/s41467-019-09106\">Cram et al. 2016 <\/a><\/p>\n\n\n\n<\/div>\n                          \n  \n                          <\/div>\n                      <div class=\"swiper-slide\">\n              <h3 class=\"pane-title\">\n                Growth rate predictions from metagenomic data\n              <\/h3>\n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>Maximum growth rate is a fundamental characteristic of microbial species that can give us a great deal of insight into their ecological role. The recently developed a tool, gRodon, enables the prediction of the maximum growth rate of an organism from genomic data on the basis of codon usage patterns. Our work and that of other groups suggest that such predictors can be applied to mixed-species communities in order to derive estimates of the average community-wide maximum growth rate.<\/p>\n<p>Selected publications: <a href=\"https:\/\/www.pnas.org\/doi\/10.1073\/pnas.2016810118\">Weissman, et al. 2021<\/a> and <a href=\"https:\/\/journals.asm.org\/doi\/10.1128\/msystems.00745-22\">Weissman, et al. 2022<\/a><\/p>\n\n\n\n<\/div>\n                          \n  \n                          <\/div>\n                      <div class=\"swiper-slide\">\n              <h3 class=\"pane-title\">\n                MIM: Machine Learning, Systems Modeling, and Experimental Approaches to Understand the Universal Rules of Life of Microbiota Using Marine Time Series Data\n              <\/h3>\n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>This project, led by PI Fengzhu Sun, aims to integrate models and field data (time series as well as global distributions) to determine how to best capture the fundamental processes controlling microbial community composition and activity, and their variation in time and space. We are closely collaborating with Dr. Sun&#8217;s group as well as co-PI Emily Zakem.<\/p>\n\n\n\n<\/div>\n                          \n  \n                          <\/div>\n                      <div class=\"swiper-slide\">\n              <h3 class=\"pane-title\">\n                Metagenomics and Metatranscriptomics\n              <\/h3>\n                                  \n<div class=\"f--field f--description\">\n\n    \n  <p>Our time-series samples offer unique opportunities to \u201cgo back in time\u201d and re-analyze archived material using the most modern techniques. Using metagenomics and bioinformatics tools such as <a href=\"http:\/\/merenlab.org\/projects\/anvio\/\">Anvi\u2019o<\/a>, we are addressing questions such as \u201cHow do genomic variants change over time?\u201d in ways that can resolve these changes at the gene or allele level.<\/p>\n<p>In concert with metagenomics, we are also mapping parallel metatranscriptomes to observe changing patterns of gene expression between adjacent sites and also over seasons. better understand what functions particular organisms are performing under different conditions.<\/p>\n<p>Reference\u00a0 <a href=\"https:\/\/doi.org\/10.1038\/s41467-019-09106\">Sieradzki et al 201<\/a>9<\/p>\n\n\n\n<\/div>\n                          \n  \n                          <\/div>\n                  <\/div>\n      <\/div>\n      <\/div>\n  \n<\/div>\n\n\n\n  <div class=\"background-images\">\n          <div class=\"swiper swiper-background-images\">\n        <div class=\"swiper-wrapper\">\n                      <div 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\n<div class=\"f--field f--image\">\n\n    \n    \n    \n    \n    \n    \n              \n      <img decoding=\"async\"\n                            src=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg\"\n          srcset=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1920x1080.jpg 1920w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1280x720.jpg 1280w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg 768w\"          sizes=\"(min-width:1200px) 75vw, (min-width:768px) 83vw, 100vw\"\n        \n                  role=\"none\"\n        \n        \n                                      \/>\n\n    \n    \n  \n  \n\n<\/div>\n                          <\/div>\n\n                      <div class=\"swiper-slide\">\n                                                                  \n<div class=\"f--field f--image\">\n\n    \n    \n    \n    \n    \n    \n              \n      <img decoding=\"async\"\n                            src=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg\"\n          srcset=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1920x1080.jpg 1920w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1280x720.jpg 1280w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg 768w\"          sizes=\"(min-width:1200px) 75vw, (min-width:768px) 83vw, 100vw\"\n        \n                  role=\"none\"\n        \n        \n                                      \/>\n\n    \n    \n  \n  \n\n<\/div>\n                          <\/div>\n\n                      <div class=\"swiper-slide\">\n                                                                  \n<div class=\"f--field f--image\">\n\n    \n    \n    \n    \n    \n    \n              \n      <img decoding=\"async\"\n                            src=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg\"\n          srcset=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1920x1080.jpg 1920w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1280x720.jpg 1280w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg 768w\"          sizes=\"(min-width:1200px) 75vw, (min-width:768px) 83vw, 100vw\"\n        \n                  role=\"none\"\n        \n        \n                                      \/>\n\n    \n    \n  \n  \n\n<\/div>\n                          <\/div>\n\n                      <div class=\"swiper-slide\">\n                                                                  \n<div class=\"f--field f--image\">\n\n    \n    \n    \n    \n    \n    \n              \n      <img decoding=\"async\"\n                            src=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg\"\n          srcset=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1920x1080.jpg 1920w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-1280x720.jpg 1280w,https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/gal2-768x432.jpg 768w\"          sizes=\"(min-width:1200px) 75vw, (min-width:768px) 83vw, 100vw\"\n        \n                  role=\"none\"\n        \n        \n                                      \/>\n\n    \n    \n  \n  \n\n<\/div>\n                          <\/div>\n\n                  <\/div>\n      <\/div>\n      <\/div>\n\n\n\n  <\/div><\/div>\n\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--spacer \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--spacer\"\n    \n      >\n\n    \n\n  <\/div><\/div>\n\n\n\n    \n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--link-list \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--link-list\"\n    \n      >\n\n    \n      <div class=\"header-container\">\n\n                  \n<div class=\"f--field f--section-title\">\n\n    \n  <h2>\n          Recent Work and Data Availabilty\n      <\/h2>\n\n\n<\/div>\n                        \n<div class=\"f--field f--description\">\n\n    \n  <p>An overview of recent work in our lab can be found on this poster from the 2022 Marine Microbes Gordon Conference.<\/p>\n<p>Much of the lab&#8217;s published data is publicly available through BCO-DMO.<\/p>\n\n\n\n<\/div>\n          <\/div>\n  \n      <div class=\"links-container\">\n\n                        \n        \n          \n            <div class=\"column\">\n                <ul>\n                                                                  <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/dornsife.usc.edu\/fuhrmanlab\/wp-content\/uploads\/sites\/207\/2023\/09\/GRC_Poster_JF_2022_42x42_print.pdf \n  target=\"_blank\"  aria-label=\"Read more about Gordon Conference Poster\"  \n>\n    Gordon Conference Poster \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                          <\/ul>\n            <\/div>\n\n          \n          \n        \n          \n            <div class=\"column\">\n                <ul>\n                                                                  <li>\n                                \n<div class=\"f--field f--link\">\n\n    \n    \n  \n<a \n  class=\"link\"\n  href= https:\/\/www.bco-dmo.org\/person\/51136\n  target=\"_blank\"  aria-label=\"Read more about Fuhrman BCO-DMO Page\"  \n>\n    Fuhrman BCO-DMO Page \n  <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 35 35\" enable-background=\"new 0 0 35 35\" width=\"25\" height=\"25\" xml:space=\"preserve\"><polygon fill-rule=\"evenodd\" clip-rule=\"evenodd\" fill=\"#000\" points=\"19.3,27.5 29.3,17.5,19.3,7.5 16.3,10.4 21.4,15.4 6.7,15.4 6.7,19.6 21.4,19.6 16.3,24.6 \"\/><\/svg>\n<\/a>\n\n\n<\/div>\n\n                                                    <\/li>\n                                                          <\/ul>\n            <\/div>\n\n          \n          \n        \n\n\n\n    <\/div>\n  \n\n  <\/div><\/div>\n\n\n\n\n\n\n      \n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--accordions \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--accordions\"\n    \n      >\n\n    \n      <div class=\"header-container\">\n\n                  \n<div class=\"f--field f--section-title\">\n\n    \n  <h2>\n          Archival Projects\n      <\/h2>\n\n\n<\/div>\n      \n      \n      \n    <\/div>\n  \n      <ul>\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-1-JC4mJCkrUP\" aria-controls=\"section-1-1-JC4mJCkrUP\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">Human impacts<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-1-JC4mJCkrUP\" role=\"region\" aria-labelledby=\"heading-1-1-JC4mJCkrUP\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <p>To study how anthropogenic inputs affect the microbial community composition and function, we analyze samples from the Port of Los Angeles, with from the SPOT site \u00a0and also a nearshore site near Catalina Island as minimally impacted controls. Using a variety of tools, we are assessing the resistance of the naturally occurring community to pollutants like heavy metals and various aromatic hydrocarbons.<\/p>\n<p>We also apply a cutting edge combination of Stable Isotope Probing (SIP) and high throughput sequencing to reveal which of the community members can metabolize pollutants and incorporate them into their biomass, directly linking diversity and function.<\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-2-JC4mJCkrUP\" aria-controls=\"section-1-2-JC4mJCkrUP\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">Archaea<\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-2-JC4mJCkrUP\" role=\"region\" aria-labelledby=\"heading-1-2-JC4mJCkrUP\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <p>We have fully sequenced one such culture, named Candidatus <em>Nitrosomarinus catalina<\/em> SPOT01, a novel strain that is less warm-temperature tolerant than other cultivated Thaumarchaeota. Using metagenomic recruitment, strain SPOT01 comprises a major portion of Thaumarchaeota (4\u201354%) in temperate Pacific waters.<\/p>\n<p>We are currently investigating archaeal global distributions and virus infectivity, among other aspects.<\/p>\n<p>Reference: <a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.13768\/full\">Ahlgren et al. 2017<\/a><\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n              <li>\n          <button type=\"button\" class=\"accordion-trigger \" id=\"heading-1-3-JC4mJCkrUP\" aria-controls=\"section-1-3-JC4mJCkrUP\" aria-expanded=\"false\" aria-disabled=\"false\">\n                          <span class=\"item-title\">Ecological Species and \u201cMicrodiversity\u201d <\/span>\n            \n                      <\/button>\n\n          <div id=\"section-1-3-JC4mJCkrUP\" role=\"region\" aria-labelledby=\"heading-1-3-JC4mJCkrUP\" class=\"accordion-panel\">\n\n                            \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n      \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <p>A major issue in microbial ecology research is how to best cluster similarly-functioning related organisms together and how to split ones that are related but ecologically different. This is essentially trying to define ecological species. While microbiologists frequently use marker genes and apply standard cutoffs of similarity, e.g. 99% similar 16S rRNA (or, 97%, which we consider too coarse), it is not clear what levels are truly most appropriate for ecological research, and how it may differ depending on the environment and scientific questions at hand.<\/p>\n<p>By taking advantage of the most recent sequencing capabilities, and combining time-series data and information from the literature and major databases, we are able to address questions relating to suitable genes for study and the level of resolution needed to relate diversity to ecological processes without excessive lumping or splitting. These are not expected to be the same for all questions and for all organisms. For example, we expect that resource utilization may be studied at a coarser sequence resolution than viral susceptibility. \u00a0This work uses both marker genes and metagenomes.<\/p>\n<p>Reference: <a href=\"http:\/\/www.nature.com\/ismej\/journal\/v11\/n7\/full\/ismej201729a.html\">Needham et al 2017<\/a><\/p>\n\n\n\n<\/div>\n\n\n  <\/div><\/div>\n            \n                      <\/div>\n        <\/li>\n\n          <\/ul>\n  \n  \n\n  <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":623,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-content-detail.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-443","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Research - Fuhrman Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/dornsife.usc.edu\/fuhrmanlab\/research\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Research - 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