{"id":194,"date":"2024-09-25T10:12:49","date_gmt":"2024-09-25T17:12:49","guid":{"rendered":"https:\/\/dornsife.usc.edu\/bridgecellculture\/?page_id=194"},"modified":"2025-08-15T12:20:16","modified_gmt":"2025-08-15T19:20:16","slug":"services","status":"publish","type":"page","link":"https:\/\/dornsife.usc.edu\/bridgecellculture\/services\/","title":{"rendered":"Services"},"content":{"rendered":"\n\n \n \n\n\n\n\n\n\n\n
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\"\"
\nWith our proven track records in G protein-coupled receptor and other protein expression and analysis, we offer following services:<\/span><\/p>\n

Baculovirus Expression and Analysis:<\/strong><\/div>\n
    \n
  • Insect cell transfection with bacmid (pFASTbac based vector)<\/strong><\/li>\n
  • Small scale expression: 40 ml, 250 ml<\/strong><\/li>\n
  • Large scale expression: 1L, 2L, 3L<\/strong><\/li>\n
  • Baculovirus generation and titer<\/strong><\/li>\n<\/ul>\n

     <\/p>\n

    Baculovirus expression is done with Sf9 suspension cells.
    \nLog phase cells are infected with titered baculovirus.
    \nAfter incubation, protein expression levels are measured with labeled FLAG antibody using Guava\u00ae <\/sup>easyCyte Flow Cytometers. Through this GPCR specific assay, we can differentiate expression level on both the cell membrane and inside of cells.<\/span><\/p>\n

     <\/p>\n

    Mammalian Expression and Analysis:<\/strong><\/div>\n
      \n
    • Transfection with purified DNA<\/strong><\/li>\n
    • Small Scale expression: 30 ml, 200 ml<\/strong><\/li>\n
    • Large scale expression: 1L, 2L, 3L<\/strong><\/li>\n<\/ul>\n

       <\/p>\n

      Mammalian expression is typically done using suspension HEK 293F cells, HEK 293Expi.
      \nLog phase cells are transfected with a CMV based expression plasmid.
      \nAfter incubation, protein expression levels are measured with labeled antibody using Guava\u00ae easyCyte Flow Cytometers. Through this GPCR specific assay, we can differentiate expression level on both the cell membrane and inside of cells.<\/span><\/p>\n

       <\/p>\n

      Cell Maintenance:<\/strong><\/div>\n
        \n
      • Weekly and biweekly passaging<\/strong><\/li>\n
      • Cell Plating for various projects<\/strong><\/li>\n
      • Cell banking<\/strong><\/li>\n
      • Cell storage<\/strong><\/li>\n
      • Media preparation<\/strong><\/li>\n
      • Quality Control Assays to monitor cell health<\/strong><\/li>\n
      • Mycoplamsa Testing<\/strong><\/li>\n<\/ul>\n

         <\/p>\n

        Consulting:<\/strong><\/div>\n
          \n
        • Cell Culture lab set up<\/strong><\/li>\n
        • Cell Culture trainings<\/strong><\/li>\n
        • Cell Culture advisement on projects<\/strong><\/li>\n<\/ul>\n

           <\/p>\n

          Current Cell Lines:<\/strong><\/div>\n

          Got Cells? Need Cells?<\/strong>
          \nCells not on this list? Please reach out to discuss the banking of any BSL1 or 2 lines, after sterility clearance.<\/strong><\/p>\n

          \"\"<\/p>\n\n\n\n<\/div>\n\n\n <\/div><\/div>\n\n\n\n\n \n \n\n\n\n\n\n\n\n

          \n\n \n
          \n
          \n \n \n
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          \n Additional Info:\n <\/h2>\n\n\n<\/div>\n \n
          \n\n \n

          Our services in baculovirus and mammalian expression are comprehensive and can be combined with other services that The Bridge offers, from cloning to expression all the way through to protein purification and quantitation. <\/p>\n

          Our services are competitive in pricing and our expertise makes for a rapid turnaround time. <\/p>\n

          All our data are databased and archived and easily available in spreadsheet format.<\/p>\n\n\n\n<\/div>\n <\/div>\n \n <\/div>\n\n\n\n <\/div><\/div>\n\n\n\n\n \n \n\n\n\n\n\n\n\n

          \n\n \n \n
          \n\n \n

          \"\"<\/p>\n\n\n\n<\/div>\n\n\n <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":89,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-194","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nServices - The Bridge Cell Culture Core<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/dornsife.usc.edu\/bridgecellculture\/services\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Services - The Bridge Cell Culture Core\" \/>\n<meta property=\"og:url\" content=\"https:\/\/dornsife.usc.edu\/bridgecellculture\/services\/\" \/>\n<meta property=\"og:site_name\" content=\"The Bridge Cell Culture Core\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-15T19:20:16+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/dornsife.usc.edu\/bridgecellculture\/services\/\",\"url\":\"https:\/\/dornsife.usc.edu\/bridgecellculture\/services\/\",\"name\":\"Services - 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