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The following instrumentation is housed within the Feakins Lab at USC Freeze-drying sediments We use a Virtis 2k freeze drier, including secondary cold trap (organic clean) to dry sediment and plant tissue prior to solvent extraction.Cryogenic Vacuum Line Extraction We have a vacuum line and offline batch distillation apparatus, to enable efficient extraction of plant leaf waters. Solvent Extraction We use Accelerated Solvent Extraction (ASE) by Dionex Corporation, which completes solvent extraction and filtration under high temperature (100°C) and pressure (1500psi).GC-MS – Gas Chromatography Mass Spectrometry We use an Agilent 6890 GC equipped with a Rxi®-5ms (30m x 0.25mm, film thickness 0.25mm); and a 7683 programmable injector connected to an Agilent 5973 MSD mass spectrometer. The Agilent GC-MS provides mass spectral information for biomarker identification and quantification. GC-IRMS – Gas Chromatography Isotope Ratio Mass Spectrometry We use a Thermo Scientific Trace GC equipped with a Rxi®-5ms (30m x 0.25mm, film thickness 1mm); and a TriPlus programmable injector connected via GC Isolink with parallel combustion furnace (at 1030 °C) and pyrolysis furnace (at 1430 °C) together with a Conflo IV to a Delta V Plus mass spectrometer. Combustion is used for the analysis of 13C/12C and pyrolysis is used for the analysis of D/H. Find IRMS resources that we have made available here. EA-IRMS – Elemental Analyzer & Isotope Ratio Mass Spectrometry We use a Costech EA in standalone mode or coupled via a Conflo IV to a Delta V Plus mass spectrometer for TC, TOC, TIC, TN, C/N, d13C TC, d13C TOC, d13C TIC, d15N TC.
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