Genome Stability and Instability and the Repair of Chromosome Breaks

Dr. James Haber, Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University
  • Date:
    Friday, April 18, 2014
  • Time:
    12:00 PM
  • Campus:
    University Park Campus
  • Venue:
    Ray R. Irani Hall (RRI)
  • Room:
    101
  • Phone:
    213-821-4464

Description:

Although our cells experience several chromosome breaks each time they replicate their DNA, the genome is remarkably stable – with the notable exception of cancer cells and other disease states that show many chromosomal rearrangements, truncations and copy number variation.  Using budding yeast as a model system, we have explored the fidelity – and infidelity – of repairing double-strand chromosome breaks induced by a site-specific endonuclease.  We find that the repair process is surprisingly prone to specific kinds of replication-associated errors leading to template switches ranging from simple frame shifts to dramatic interchromosomal template switches and microhomology-mediated rearrangements.

  • Department of Biological Sciences
  • University of Southern California
  • Allan Hancock Foundation Building
  • Los Angeles, CA 90089-0371